Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Dual specificity and target gene selection by the MADS domain protein FRUITFULL

ABSTRACT: Dual specificity and target gene selection by the MADS domain protein FRUITFULL

PROVIDER: PRJNA820961 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR18512070.fastq.gz	Fastqsanger.gz
	SRR18512071.fastq.gz	Fastqsanger.gz
	SRR18512072.fastq.gz	Fastqsanger.gz
	SRR18512073.fastq.gz	Fastqsanger.gz
	SRR18512074.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 28

Similar Datasets

Dual specificity and target gene selection by the MADS domain protein FRUITFULL

Project description:How transcription factors of a single family confer different functional specificities in vivo, is an important question in molecular biology. Even more intriguingly, a single transcription factor can regulate context- or tissue-specific target genes to achieve distinct functions. Here we show, using a variety of genome-wide techniques, that gene regulation and DNA binding site selection by the MADS domain protein FRUITFULL (FUL) is tissue-specific. FUL has a dual role in regulating floral transition and fruit development.

2022-10-04 | GSE199643 | GEO

Dual specificity and target gene selection by the MADS domain protein FRUITFULL

2022-10-03 | GSE194055 | GEO

Dual specificity and target gene selection by the MADS domain protein FRUITFULL

Project description:Dual specificity and target gene selection by the MADS domain protein FRUITFULL

| PRJNA798963 | ENA

Genome-wide mapping of binding sites of the MADS domain protein FRUITFULL in Arabidopis thaliana

Project description:Genome-wide mapping of binding sites of the MADS domain protein FRUITFULL in Arabidopis thaliana

| PRJNA427320 | ENA

Differences in DNA-binding specificity of floral homeotic protein complexes predict organ-specific target genes

Project description:Floral organ identities in plants are specified by the combinatorial action of homeotic master regulatory transcription factors (TFs). How these factors achieve their regulatory specificities is however still largely unclear. Genome-wide in vivo DNA binding data show that homeotic MADS-domain proteins recognize partly distinct genomic regions, suggesting that DNA binding specificity contributes to functional differences of homeotic protein complexes. We used in vitro systematic evolution of ligands by exponential enrichment followed by high throughput DNA sequencing (SELEX-seq) on several floral MADS-domain protein homo- and heterodimers to measure their DNA-binding specificities. We show that specification of reproductive organs is associated with distinct binding preferences of a complex formed by SEPALLATA3 (SEP3) and AGAMOUS (AG). Binding specificity is further modulated by different binding site (BS) spacing preferences. Combination of SELEX-seq and genome-wide DNA binding data allows to differentiate between targets in specification of reproductive versus perianth organs in the flower. We validate the importance of DNA-binding specificity for organ-specific gene regulation by modulating promoter activity through targeted mutagenesis. Our study shows that intrafamily protein interactions affect DNA-binding specificity of floral MADS-domain proteins. DNA-binding specificity of individual dimers, as well as DNA-binding preferences of higher-order complexes differ between floral homeotic protein complexes. Differential DNA-binding of MADS-domain protein complexes plays a role in the specificity of target gene regulation.

2017-10-16 | GSE95730 | GEO

SPOROCYTELESS/NOZZLE interacts with MADS-DOMAIN transcription factors during the megaspore mother cell differentiation in the Arabidopsis ovule

Project description:This dataset contains proteomic data from co-immunoprecipitation and mass spectrometry (IP-MS) experiments performed to identify in planta interaction partners of the transcriptional regulator SPOROCYTELESS/NOZZLE (SPL) during Arabidopsis thaliana ovule development. SPL is an essential regulator of megaspore mother cell (MMC) specification, yet the protein complexes through which it acts remained largely unknown. To elucidate the SPL interactome, a pSPL:SPL-GFP fusion was expressed in the pAP1:AP1-GR ap1 cal floral inducible system, allowing synchronized flower development and enrichment of ovule stages preceding MMC formation. SPL-GFP protein complexes were isolated from inflorescences at early ovule developmental stages and analysed by LC-MS/MS. The IP-MS analysis revealed 660 proteins significantly enriched in SPL-GFP immunoprecipitates compared with input controls, providing the first comprehensive view of SPL-associated nuclear partners in reproductive tissues. Among transcription factors co-purifying with SPL, MADS-domain proteins were particularly prominent, representing about one fifth of all enriched TFs. These included SEEDSTICK (STK), SHATTERPROOF2 (SHP2), SEPALLATA1-3 (SEP1–3), and AGAMOUS (AG)—core components of the ovule identity regulatory network. The detection of these ovule-specific MADS-domain transcription factors among SPL interactors strongly supports a model in which SPL is recruited into multimeric MADS-domain complexes that coordinate nucellus development and MMC specification. The SPL IP-MS dataset therefore provides crucial insight into the protein interaction landscape controlling female germline initiation in Arabidopsis. It identifies SPL as a central component of ovule identity complexes and reveals a biochemical connection between SPL activity and the MADS-mediated transcriptional network that defines nucellar fate and auxin-dependent MMC differentiation.

2025-11-18 | PXD070409 | Pride

The Function of Tetrameric MADS-box Transcription Factors in Floral Fate Determination [RNA-seq]

Project description:MADS-domain transcription factors (TF) are critical in determining floral cell fate and floral organ identities. However, their function molecular mechanisms are still poorly understood. Here, we show that effective DNA-binding in closed chromatin and target gene regulation of APETALA1 (AP1), a key floral MADS-box TF requires tetramerisation, indicating the fundamental role of tetrameric MADS-box TFs in floral cell fate programming.

2025-11-13 | GSE227950 | GEO

The Function of Tetrameric MADS-box Transcription Factors in Floral Fate Determination [ATAC-seq]

2025-11-13 | GSE227949 | GEO

The Function of Tetrameric MADS-box Transcription Factors in Floral Fate Determination [ChIP-seq]

2025-11-13 | GSE227948 | GEO

The Function of Tetrameric MADS-box Transcription Factors in Floral Fate Determination [Mnase-seq]

2025-11-13 | GSE297049 | GEO