Ontology highlight
ABSTRACT:
PROVIDER: PRJNA821062 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR18516326_1.fastq.gz | Fastqsanger.gz | |||
| SRR18516326_2.fastq.gz | Fastqsanger.gz | |||
| SRR18516327_1.fastq.gz | Fastqsanger.gz | |||
| SRR18516327_2.fastq.gz | Fastqsanger.gz | |||
| SRR18516328_1.fastq.gz | Fastqsanger.gz |
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Project description:Streptococcus pyogenes (Group A Strep, GAS) is a serious human pathogen with the ability to colonize mucosal surfaces such as the nasopharynx and vaginal tract, often leading to infections such as pharyngitis and vulvovaginitis. We present genome-wide RNASeq data showing the transcriptomic changes GAS undergoes during vaginal colonization. These data reveal that the regulon controlled by MtsR, a master metal regulator, is activated during vaginal colonization. This regulon includes two genes highly expressed during vaginal colonization, hupYZ. Here we show that HupY binds heme in vitro, affects intracellular concentrations of iron, and is essential for proper growth of GAS using hemoglobin or serum as the sole iron source. HupY is also important for murine vaginal colonization of both GAS and the related vaginal colonizer and pathogen, Streptococcus agalactiae (Group B Strep, GBS). These data provide essential information on the link between metal regulation and mucosal colonization in both GAS and GBS.
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Project description:In the transition from recto-vaginal colonizing organism to invasive pathogen, Streptococcus agalactiae (Group B Streptococcus, GBS) must adapt to changes in host temperature, including elevated temperatures due to host fever. To identify genes important to the survival of GBS in response to heat stress, transcriptional profiling was performed using DNA microarray analysis, comparing GBS grown at normal temperature (37˚C) to GBS exposed to elevated temperature (42˚C).
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