Ontology highlight
ABSTRACT:
PROVIDER: PRJNA821415 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:We aimed to validate an 18-gene classifier (GC) initially developed to predict local/regional recurrence (LRR) after mastectomy in estimating distant metastasis risk.
2017-08-11 | GSE102484 | GEO
Project description:We report an upregulation of miR-16 in Paget's disease of bone which is to target the SQSTM1. When miR-16 is lost in Paget's disease, there is malignant transformation.
2017-04-18 | GSE87018 | GEO
Project description:Tattoos are becoming increasingly popular across generations, with 10-20% of the population in Europe and the United States bearing at least one tattoo. Tattoos represent an intradermal injection of pigments (i.e. coloured insoluble micro- and nano-particles), which remain in place throughout life. Although tattoos have existed for centuries, the question of their health impact has really arisen rather lately, so that a significant gap in our understanding of the cellular and molecular long-term effects of these pigments on the body remains. In this project, we investigate a wide range of tattoo pigments on macrophages. Macrophages, pivotal in pigment persistence through phagocytic processes and capture-release-recapture cycles, are also key players in the inflammatory response and tissue homeostasis. Dysregulation in macrophage functionalities can potentially lead to substantial health consequences such as deficient immune response or such as sarcoidosis. To assess the long-term effects of tattoos, we tested iron-based pigments (which are widely used in the medical field for dermopigmentation of the nipple-areola complex after mastectomy) using an exposure scheme consisting in an acute exposure for 24 hours and a 5-days post exposure recovery period. Proteomic analyses were conducted to understand the underlying cellular mechanisms.
2025-10-09 | PXD064985 | Pride
Project description:Molecular Determinants of Post-Mastectomy Breast Cancer Recurrence
| PRJNA490741 | ENA
Project description:We extracted total RNA from fingerstick samples of normal, chondrosarcoma and Paget's disease patients. We then constructed small RNA libraries using HD adapters in order to identify small RNA biomarkers
2021-08-13 | GSE87054 | GEO
Project description:The nipple is an example of specialized epidermis. The unique epidermal stratification and gene expression is induced and maintained by developmental distinct fibroblast populations. To identify specific signaling modules involved in the maintenance of nipple epidermis we used gene expression profiling of purified fibroblast populations from a murine model of nipple skin formation
2017-03-13 | GSE87030 | GEO
Project description:Purpose: Several risk factors for local recurrence of breast cancer after breast conserving therapy (BCT) have been identified. The identification of additional risk factors would be very useful in guiding optimal therapy and also improve understanding of the mechanisms underlying local recurrence. We used cDNA microarray analysis to identify gene expression profiles associated with local recurrence. Experimental Design: Using 18K cDNA microarrays, gene expression profiles were obtained from 50 patients who underwent BCT. Of these 50 patients 19 developed a local recurrence; the remaining 31 patients were selected as controls as they were free of local recurrence at least 11 years after treatment. For 9/19 patients also the local recurrence was available for gene expression profiling. Unsupervised and supervised methods of classification were used to separate patients in groups corresponding to disease outcome and to study the overall gene expression pattern of primary tumors and their recurrences. Results: Hierarchical clustering of patients did not show any grouping reflecting local recurrence status. Supervised analysis revealed no significant set of genes that was able to distinguish recurring tumors from non-recurring tumors. Paired-data analysis of primary tumors and local recurrences showed a remarkable similarity in gene expression profile between primary tumors and their recurrences. Conclusions: No significant differences in gene expression between primary breast cancer tumors in patients with or without local recurrence after breast conserving therapy were identified. Furthermore, analyses of primary tumors and local recurrences show a preservation of the overall gene expression pattern in the local recurrence, even after radiotherapy. Keywords: gene expression profiling
2006-09-24 | GSE4913 | GEO
Project description:Molecular Determinants of Post-Mastectomy Breast Cancer Recurrence
2018-09-20 | PRJEB28702 | EVA
Project description:Background: While body fluids are widely used in medicine, despite the fact that interstitial fluid (IF) represents a third of our body fluid, it is the most poorly understood and under-studied. In cancer, IF pressure is elevated due to increased extracellular matrix deposition in the desmoplastic stroma surrounding tumors. However, whether IF affects cancer behavior remains unclear. Methods: We collected 180 IF using drains from patients that underwent bilateral mastectomy for unilateral breast cancer with or without immediate reconstruction. The IF were classified based on their ability to promote invasion of breast cancer cells and tested in vivo. A retrospective study linking IF-invasion score to clinical parameters was performed. Results: We observed a wide range of invasion score among IF between patients, and analysis of different IF from the same patient suggests that IF are organ-specific. Patients with high-grade tumors at diagnosis have high score IF. In mice, injections of high-score IF in a normal mammary gland promoted ductal hyperplasia, increased collagen deposition and local invasion. In a mouse model of residual disease, high-score IF increased local recurrence and promoted aggressive visceral metastases. Discussion: Exposure of normal breasts to high-score IF predisposes to the development of aggressive breast cancer long before surgery. Further, such exposure following surgery increases the risk of recurrence. Therefore, our study raises the provocative possibility that intrinsic difference in IF between individuals play an active role in the predisposition, development and clinical course of sporadic breast cancers.
2018-02-01 | GSE77812 | GEO