Project description:Semi-field translocations to examine whether gene expression differences between Anopheles gambiae M and S occupying discrete larval habitats are due to inherent molecular form divergence or transcriptional plasticity
Project description:Temperature is an important factor that affects the growth and reproduction of mangrove plants. Laguncularia racemosa (L.) C.F. Gaertn is a controversial species in China, in terms of being a pioneer species for mangrove restoration and a putative invasive species occupying the natural habitats. One of its advantages is the strong resistance to low temperature, which makes L. racemose more adaptive to extreme climate change than local mangrove species. However, little is known about the regulatory mechanisms underlying the cold-stress tolerance in L. racemose, which restricted our understanding on its biological features and invasion potential. In this study, In the current study, we investigated the physiological and molecular mechanisms involved in chilling-stress adaptation in L. racemose. Freezing temperature caused damage to cell membrane system and reduced photosynthesis efficiency in L. racemose. To combat the adverse impacts, plasma membrane biosynthesis and antioxidant processes were substantially enhanced. Furthermore, we showed that there was a difference between the responses to freeze-thaw injury in L. racemose from different locations. It may provide new clues to the different genetic background between varieties of L. racemose. These novel findings could provide biochemical and genetic basis for the cultivation and restoration of L. racemose.
Project description:Iron-sulfur minerals such as pyrite are found in many marine benthic habitats. At deep-sea hydrothermal vent sites they occur as massive sulfide chimneys. Hydrothermal chimneys formed by mineral precipitation from reduced vent fluids upon mixing with cold oxygenated sea water. While microorganisms inhabiting actively venting chimneys and utilizing reduced compounds dissolved in the fluids for energy generation are well studied, only little is known about the microorganisms inhabiting inactive sulfide chimneys. We performed a comprehensive meta-proteogenomic analysis combined with radiometric dating to investigate the diversity and function of microbial communities found on inactive sulfide chimneys of different ages from the Manus Basin (SW Pacific). Our study sheds light on potential lifestyles and ecological niches of yet poorly described bacterial clades dominating inactive chimney communities.
Project description:In this study, the transcriptional profiles of four deep-sea sculpin fish species (Cottoidei) are described for the first time as part of the study of the adaptation of endemic fishes to different habitats in Lake Baikal. These studies will lead to a deeper understanding of the molecular mechanisms that ensure the adaptation of endemic species to specific habitats in Lake Baikal, even at depths of more than 500 m (up to 1600 m).
2025-10-30 | GSE308109 | GEO
Project description:Microbiome of CCA species from different habitats
Project description:Lactobacillus casei is remarkably adaptive to diverse habitats. To understand the evolution and adaptation of Lb. casei strains isolated from different environments, the gene content of 22 Lb. casei strains isolated from various habitats (cheeses, n=8; plant materials, n=8; and human sources, n=6) were examined by comparative genome hybridization with an Lb. casei ATCC 334-based microarray.
Project description:Phytomonas are a large and diverse sub-group of plant-infecting trypanosomatids that are relatively poorly understood. Little is known of their biology or how they have adapted to life inside plants. This study sequenced the genome of the Cassava (Manihot esculenta) infecting species Phytomonas francai to provide additional genome resources and new insight into the biology of this poorly understood group of organisms.
Project description:The facultative chemolithoautotroph Cupriavidus necator is capable of heterotrophic growth on diverse carbon sources or of autotrophic growth using CO2 fixation with H2 as an energy source. Under stress conditions, it is capable of producing biodegradable polyesters (polyhydroxyalkanoates, PHAs) as a storage material occupying a high proportion of the total biomass. This metabolic versatility means that C. necator is under intense study for sustainable biotechnology processes; however a relative lack of understanding of the overall regulatory architecture has limited its application. The major mechanisms by which proteins can respond to shifting cellular demands are protein expression change and/or allosteric regulation. Here we use two powerful proteomics methods to investigate these responses in C. necator cells grown on balanced and low nitrogen (PHA-inducing) media. Using quantitative proteomics and protein stability analysis (which can report on conformation change), we find that proteins across different pathways respond through one or both of these regulatory modes, including coordinated adaption to nutrient stress by the PHA pathway, the Calvin cycle, and ribosomal proteins.
