Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Single-cell analysis of conventional dendritic cells in an immunogenic murine melanoma model

ABSTRACT: Single-cell analysis of conventional dendritic cells in an immunogenic murine melanoma model

PROVIDER: PRJNA823542 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Single-cell analysis of conventional dendritic cells in an immunogenic murine melanoma model

Project description:Single-cell RNA sequencing was used to show the heterogeneity of conventional dendritic cells in the immunigenic murine melanoma BRAFV600E Ptgs1/Ptgs2-/-. Our analysis shows that on day 6 of tumor growth, 3 distinct populations of cDC1 and 2 distinct populations of cDC2 can be found in the tumor microenvironment. The differentially expressed genes of these populations indicate a functional heterogeneity.

2023-07-13 | GSE200174 | GEO

Inflammatory type 2 conventional dendritic cells contribute to murine and human cholangitis

Project description:Cholangitis mouse models were characterised by selective intrahepatic expansion of type 2 conventional dendritic cells, whereas plasmacytoid and type 1 conventional dendritic cells were not expanded. Expansion of type 2 conventional dendritic cells in human PSC lesions was confirmed by histology. Depletion studies revealed a pro-inflammatory role of type 2 conventional dendritic cells. Single-cell transcriptomics confirmed inflammatory maturation of the intrahepatic type 2 conventional dendritic cells and identified dendritic cell-derived inflammatory mediators.

2022-07-08 | E-MTAB-11821 | biostudies-arrayexpress

Genome accessibility profile at single cell level for conventional dendritic cells in human metastatic melanoma samples [snATAC-Seq]

Project description:Conventional dendritic cells (cDCs) has been shown to mediate immune checkpoint inhibitor responses in cancer patients. We used single nucleus ATAC sequencing (snATAC-seq) to analyze the epigenome of heterogeneous cDCs obtained from 14 human metastatic melanoma samples.

2025-07-30 | GSE303948 | GEO

RNA sequencing of murine conventional dendritic cell lineage 1 (cDC1) cells from mesenteric lymph nodes

Project description:A gene expression profile of the conventional dendritic cell lineage 1 from murine mesenteric lymph nodes (MLN) were constructed based on RNA-seq from nine biological biopsies. The gene expression profile was afterwards used to infer (in silico) a cDC1-specific protein-protein interactome.

2020-07-01 | GSE150549 | GEO

Inflammatory type 2 conventional dendritic cells contribute to murine and human cholangitis

Project description:Inflammatory type 2 conventional dendritic cells contribute to murine and human cholangitis

| PRJEB53691 | ENA

Newly Found Rat CD103− Dendritic Cells Represent a Highly Immunogenic Subpopulation of Type-2 Conventional Dendritic Cells, Corresponding to Known Dendritic Cell Subsets in Mice and Humans

Project description:Newly Found Rat CD103− Dendritic Cells Represent a Highly Immunogenic Subpopulation of Type-2 Conventional Dendritic Cells, Corresponding to Known Dendritic Cell Subsets in Mice and Humans

| PRJNA1183350 | ENA

Single cell RNA sequencing reveals distinct clusters of Irf8 dependent pulmonary conventional dendritic cells

Project description:We hereby report application of single cell RNA sequencing in order to understand heterogeneity within macrophages and conventional dendritic cells found in murine lungs. In this paper we specifically show different populations of cDCs as revealed through single cell RNA sequencing

2023-05-04 | GSE195899 | GEO

In vivo dendritic cell reprogramming for cancer immunotherapy [organoid scRNA]

Project description:Immunotherapy can lead to long-term survival for some cancer patients, yet generalized success has been hampered by insufficient antigen presentation and exclusion of immunogenic cells from the tumor microenvironment. Here, we developed an approach to reprogram tumor cells in vivo by adenoviral delivery of the transcription factors PU.1, IRF8, and BATF3, which enabled them to present antigens as type 1 conventional dendritic cells. Reprogrammed tumor cells remodeled their tumor microenvironment, recruited, and expanded polyclonal cytotoxic T cells, induced tumor regressions, and established long-term systemic immunity in multiple mouse melanoma models. In human tumor spheroids and xenografts, reprogramming to immunogenic dendritic-like cells progressed independently of immunosuppression, which usually limits immunotherapy. Our study paves the way for human clinical trials of in vivo immune cell reprogramming for cancer immunotherapy.

2024-09-05 | GSE255536 | GEO

Gene expression in Mouse thrombomoudlin+ and thrombomodulin- dendritic cell

Project description:Previously we had shown in a mouse model of bronchial asthma that thrombomodulin (TM; CD141; BDCA3) can convert immunogenic conventional dendritic cells into tolerogenic dendritic cells while inducing its own expression on the cell surface. Thrombomodulin+ dendritic cells are tolerogenic while thrombomodulin- dendritic cells are pro-inflammatory and immunogenic. Here we hypothesized that thrombomodulin treatment of dendritic cells would modulate inflammatory gene expression. Murine bone marrow derived dendritic cells were treated with soluble thrombomodulin and expression of surface markers was determined. Treatment with thrombomodulin reduces the expression of maturation markers and increases the expression of TM on the DC surface. Thrombomodulin treated and control dendritic cells were sorted into thrombomodulin+ and thrombomodulin- dendritic cells before their mRNA was analyzed by microarray. mRNAs encoding pro-inflammatory genes and dendritic cells maturation markers were reduced while cell cycle genes were increased in thrombomodulin-treated and thrombomodulin+ dendritic cells compared to control dendritic cells and thrombomodulin- dendritic cells. We compared the effects on global gene expression of treating dendritic cells with soulble thrombomodulin to no treatment, and dendritic cells separated into thrombomodulin+ and thrombomodulin- dendritic cell subsets.

2014-12-31 | GSE45652 | GEO

Comparative analysis of WT and XCR1 KO type 1 conventional dendritic cells [array]

Project description:XCR1 is known as a signature receptor of type 1 conventional dendritic cells To compare the transcriptomic differences of WT and XCR1 deficient type 1 conventional dendritic cells, we conducted microarry using type 1 conventional dendritic cells from WT and XCR1 KO mice

2024-05-20 | GSE267410 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data