Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

TLE3 CUT&Tag-Seq to characterize TLE3-binding sites in colonic macrophages

ABSTRACT: TLE3 CUT&Tag-Seq to characterize TLE3-binding sites in colonic macrophages

PROVIDER: PRJNA825591 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR18725205_1.fastq.gz	Fastqsanger.gz
	SRR18725205_2.fastq.gz	Fastqsanger.gz

Items per page:

1 - 2 of 2

Similar Datasets

TLE3 CUT&Tag-Seq to characterize TLE3-binding sites in colonic macrophages

Project description:We found that TLE3 in colonic macrophages mainly occupied distal intergenic regions (48%) and gene body regions (47%) with limited occupancy at the gene proximal regions (5%).

2023-04-07 | GSE200645 | GEO

Adipose subtype-selective recruitment of TLE3 in thermogenic gene programs

Project description:Transcriptional effectors of white adipocyte-selective gene expression have not been described. TLE3 is a white-selective cofactor that acts reciprocally with the brown-selective cofactor Prdm16 to specify lipid storage and thermogenic gene programs. When expressed at elevated levels in brown fat, TLE3 counters Prdm16, suppressing brown-selective genes and inducing white-selective genes, resulting in impaired fatty acid oxidation and thermogenesis. To further test the functional consequences of the changes in BAT phenotype in aP2-TLE3 Tg mice, we challenged them with cold exposure at 4C. aP2-TLE3 Tg mice had an impaired ability to respond to cold exposure compared to littermate control mice, as evidenced by a marked drop in core body temperature. Transgenic mice expressing TLE3 from the aP2 enhancer and wild type mice were housed individually without food and bedding immediately before the start of experiments. Mice were allowed free access to water and placed at 4C for 5 hours while core body temperature was monitored every hour using a rectal probe. For microarray experiments of BAT, RNA was extracted and pooled from 4 mice in each group.

2013-09-27 | E-GEOD-51223 | biostudies-arrayexpress

H3K27Ac CUT&Tag-Seq to characterize the epigenetic profiles in colonic macrophages from Tle4KO (Tle4fl/fl, Lyz2-Cre) and WT (Lyz2-Cre) mice

Project description:H3K27Ac CUT&Tag-Seq to characterize the epigenetic profiles in colonic macrophages from Tle4KO (Tle4fl/fl, Lyz2-Cre) and WT (Lyz2-Cre) mice

| PRJNA825592 | ENA

RNA-Seq to profile transcriptomes in colonic macrophages from Tle3KO (Tle3fl/fl, Lyz2-Cre), Tle4KO (Tle4fl/fl, Lyz2-Cre) and WT (Lyz2-Cre) mice

Project description:We identified TLE3- and TLE4-regulated transcriptomes in colonic macrophages.

2023-04-07 | GSE183232 | GEO

Genome-wide mapping of MEF2A binding sites by CUT&Tag-seq in chicken primary myoblasts

Project description:In this study, CUT&Tag-seq technology was employed to investigate MEF2A binding sites across the entire genome of chicken primary myoblasts. CUT&Tag was performed using CUT&Tag Assay Kit for Illumina Pro (TD904-1) from Vazyme. Antibody targeting MEF2A as well as IgG were used.The final DNA library on a HiSeq PE150 platform was subjected for the analyses. This study provides a wide landscape of MEF2A target genes from chicken primary myoblasts, which supports the active role of MEF2A in avian muscle development.

2023-12-05 | GSE249097 | GEO

Cut&Tag of 15 week-old HD R6/1 mice in neuronal cell populations.

Project description:To characterize the epigenome of HD R6/1 mice in striatal cells using CUT&TAG on neuronal nuclei

2025-01-08 | GSE282238 | GEO

HCT116_SMARCA4 CUT&Tag

Project description:HCT116_SMARCA4 CUT&Tag

| PRJNA875063 | ENA

CUT&TAG analysis of R-loops in Mcm8 knockout MEFs and CUT&TAG analysis of R-loops and MCM8-binding sites in HEK293 cells

Project description:To reveal the role of MCM8 in suppressing R-loop accumulation, we performed the CUT&TAG assay using the S9.6 antibody to map genome-wide R-loops in Mcm8 wildtype MEFs and Mcm8 knockout MEFs. We also conducted the CUT&TAG assay to detect genome-wide R-loops in Ddx5 downregulated MEFs by adenovirus infection and in control MEFs. To investigate the underlying molecular mechanism of MCM8 suppressing R-loops, we conducted the DNA sequencing of libraries from CUT&TAG assay using the antibody against FLAG in HEK293 cells transfected with FLAG-MCM8 plasmid and using the S9.6 antibody in HEK293 cells. Besides, an IgG control and control of RNH1 overexpression were included.

2024-05-30 | GSE254352 | GEO

The histone demethylase KDM5C represses trophoblast proliferation and invasion in woman with recurrent miscarriage [CUT & Tag]

Project description:To characterize genome-wide KDM5C traget genes in HTR-8/SVneo cells transduced with KDM5C-expression vector by CUT&Tag.

2022-12-16 | GSE200540 | GEO

TLE3 and TLE4-coordinated colonic macrophage-CD4+ T cell crosstalk maintains intestinal immune homeostasis

Project description:TLE3 and TLE4-coordinated colonic macrophage-CD4+ T cell crosstalk maintains intestinal immune homeostasis

| PRJNA759763 | ENA