Ontology highlight
ABSTRACT:
PROVIDER: PRJNA827243 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR18776443_1.fastq.gz | Fastqsanger.gz | |||
| SRR18776443_2.fastq.gz | Fastqsanger.gz | |||
| SRR18776444_1.fastq.gz | Fastqsanger.gz | |||
| SRR18776444_2.fastq.gz | Fastqsanger.gz | |||
| SRR18776445_1.fastq.gz | Fastqsanger.gz |
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Project description:Morphine and its pharmacological derivatives are the most prescribed analgesics for moderate to severe pain management. However, chronic use of morphine reduces pathogen clearance and induces bacterial translocation across the gut barrier. The enteric microbiome has been shown to play a critical role in the preservation of the mucosal barrier function and metabolic homeostasis. Here, we show for the first time, using bacterial 16s rDNA sequencing, that chronic morphine treatment significantly alters the gut microbial composition and induces preferential expansion of the gram-positive pathogenic and reduction of bile-deconjugating bacterial strains. A significant reduction in both primary and secondary bile acid levels was seen in the gut, but not in the liver with morphine treatment. Morphine induced microbial dysbiosis and gut barrier disruption was rescued by transplanting placebo-treated microbiota into morphine-treated animals, indicating that microbiome modulation could be exploited as a therapeutic strategy for patients using morphine for pain management. In this study, we establish a link between the two phenomena, namely gut barrier compromise and dysregulated bile acid metabolism. We show for the first time that morphine fosters significant gut microbial dysbiosis and disrupts cholesterol/bile acid metabolism. Changes in the gut microbial composition is strongly correlated to disruption in host inflammatory homeostasis13,14 and in many diseases (e.g. cancer/HIV infection), persistent inflammation is known to aid and promote the progression of the primary morbidity. We show here that chronic morphine, gut microbial dysbiosis, disruption of cholesterol/bile acid metabolism and gut inflammation; have a linear correlation. This opens up the prospect of devising minimally invasive adjunct treatment strategies involving microbiome and bile acid modulation and thus bringing down morphine-mediated inflammation in the host.
2016-06-01 | E-MTAB-3722 | biostudies-arrayexpress
Project description:Ellagic acid is a kind of polyphenol lactone, which is widely found in various fruits and nuts. It’s the natural inhibitor of CARM1. Ellagic acid was reported to inhibit breast cancer growth and metastasis. But the mechanism of Ellagic acid has not been investivaged clearly in triple-negative breast cancer (TNBC). Our results indicate that direct inhibition of CDK4 by Ellagic acid limits breast cancer proliferation and metastasis. Our research has determined the molecular basis of Ellagic acid effect in TNBC, which may provide new ideas and drugs for cancer therapy.
2024-04-07 | GSE171618 | GEO
Project description:Background In broilers, heat stress can result in reduced feed consumption, digestive inefficiency, impaired metabolism, and even death. The broiler sector of the U.S. poultry industry incurs approximately $52 million in heat stress-related losses annually. The objective of this study is to characterize the effects of chronic, cyclic heat stress on the transcriptome of a metabolically active organ, the liver. Characterizing the liver transcriptome of heat-stressed broilers will help clarify the effects of heat stress on metabolism. This information will provide a platform for future investigations that further elucidate physiologic responses to heat stress and seek methods to ameliorate the negative impacts of heat. Results Transcriptome sequencing of the livers of 8 broiler males using Illumina HiSeq 2000 technology, resulted in a total of 138 million, 100 base pair single end reads, yielding 13.8 gigabases of sequence. Forty genes were differentially expressed at a significance level of P-value < 0.05 and a fold change ≥ 2 in response to chronic, cyclic heat stress (mid-point of the last day of a 7-day cyclic heat stress of 7 hours per day), with 27 down-regulated and 13 up-regulated. Two gene networks were created from the function-based Ingenuity Pathway Analysis (IPA) of the differentially expressed genes; “Cell Signaling, Molecular Transport, Small Molecule Biochemistry” and “Endocrine System Development and Function, Small Molecule Biochemistry Cell Signaling”. Members of the MAPK signaling pathway and differentially expressed genes that are associated with MAPK-related functions were prominent in the networks. Cellular proliferation and differentiation, inflammationand stress-related signaling, and apoptosis-associated genes were down-regulated in response to heat stress. Genes responsible for inhibiting feed intake and sphingolipidrelated signaling were up-regulated. Genes involved with the regulation of inflammation, stress, thyroid hormone level, and body temperature were both up- and down-regulated. Conclusions Chronic, cyclic heat stress of broilers results in metabolic changes that can be characterized through RNA-seq analysis of the liver transcriptome. The primary affected pathways included cell signaling, molecular transport, endocrine system development and signaling, and small molecule biochemistry. Examination of 2 heat treatments. Four heat stressed liver samples and 4 control liver samples analyzed.
2013-09-20 | E-GEOD-51035 | biostudies-arrayexpress
Project description:To examine the mechanism underlying chemoprevention and changes in gene expression pattern induced by chlorophyllin and ellagic acid during 7,12-dimethylbenz[a]anthracene (DMBA)-induced hamster buccal pouch (HBP) carcinogenesis Two-condition experiment, Control vs. DMBA, Control vs. DMBA+chloophyllin, Control vs. DMBA+ellagic acid. Biological replicates: 2 control replicates, 2 DMBA replicates, 2 DMBA+chlorophyllin replicates, 2 DMBA+ellagic acid replicates.
2011-06-02 | E-GEOD-29679 | biostudies-arrayexpress
Project description:The black nectar of Melianthus flowers is thought to serve as a visual attractant to pollinators, but the chemical identity and synthesis of the black pigment are unknown. Here we report that the black nectar contains a natural analog of iron-gall ink, which humans have used since medieval times. Specifically, dark black nectar at anthesis contains high levels of ellagic acid and iron; synthetic solutions of ellagic acid and iron(III) recapitulate the black color of the nectar. Conversely, lightly colored nectars before and after anthesis contain significantly lower levels of ellagic acid and iron, but higher levels of gallic acid. We then explored the possibility of post-secretory synthesis of ellagic acid from gallic acid. Indeed, Melianthus nectar contains a peroxidase that oxidizes gallic acid to form ellagic acid. Reactions containing the nectar peroxidase, gallic acid, hydrogen peroxide, and iron can fully recreate the black color of the nectar. Visual modeling indicates that the black color is both visible and conspicuous to birds within the context of the flower. In summary, the black nectar of Melianthus is derived from an ellagic acid-Fe complex analogous to iron-gall ink and is likely involved in the attraction of passerine bird pollinators.
2023-01-01 | GSE221730 | GEO
Project description:<p>Immune stress in broilers can cause severe economic losses, chlorogenic acid is an effective plant extract for alleviating immune stress. This study investigates the effect of chlorogenic acid on the intestinal microbiota and metabolites of broilers under immune stress. 312 broilers were randomly divided into 4 treatment groups, namely saline group, lipopolysaccharides (LPS) group, saline+chlorogenic acid (SCGA) group, and LPS+CGA (LCGA) group. The LPS group and LCGA group of broilers were injected with 0.5 mg/kg LPS intraperitoneally at 14, 15, and 17 days of age to induce immune stress. SCGA and LCGA group fed a basal diet supplemented with 1000 mg/kg CGA. At 17d, the ileal contents of broilers were collected for microbial and metabolomics analysis. Results showed that immune stress led Subdoligranulum decreased, thereby lowering the diversity of the intestinal microbial community. CGA increase the abundance of beneficial bacteria Clostridiaceae and Candidatus Arthromitus in the ileum of immune-stressed broilers, while reducing the abundance of Lachnospiraceae, Allobaculum, Desulfovibrio, Desulfovibrionales, Desulfovibrionaceae. Non-targeted metabolomics results showed that immune stress significantly increased the content of methylmalonic acid, N-Acetyl-L-aspartic acid, trans-ferulic acid, and pantothenic acid in the ileum of broilers, while significantly downregulated the content of pyroglutamic acid, L-glutamic gamma-semialdehyde, and biliverdin, indicating that LPS activated inflammation and oxidation in broilers, leading to oxidative damage in the intestine. After CGA added, the levels of corticosterone, prostaglandin B2, trans-ferulic acid, methylmalonic acid in the ileum of immune-stressed broilers were significantly lower, while the levels of pyroglutamic acid, L-glutamic gamma-semialdehyde, biliverdin, chenodeoxycholic acid, desmosterol, and 4-hydroxycinnamic acid were significantly higher, indicating that CGA can alleviate the inflammatory response induced by immune stress and enhance antioxidant capacity. Therefore, CGA can alleviate immune stress by regulating the ileal microbiota and metabolites.</p>
2025-06-09 | MTBLS11790 | MetaboLights
Project description:Background In broilers, heat stress can result in reduced feed consumption, digestive inefficiency, impaired metabolism, and even death. The broiler sector of the U.S. poultry industry incurs approximately $52 million in heat stress-related losses annually. The objective of this study is to characterize the effects of chronic, cyclic heat stress on the transcriptome of a metabolically active organ, the liver. Characterizing the liver transcriptome of heat-stressed broilers will help clarify the effects of heat stress on metabolism. This information will provide a platform for future investigations that further elucidate physiologic responses to heat stress and seek methods to ameliorate the negative impacts of heat. Results Transcriptome sequencing of the livers of 8 broiler males using Illumina HiSeq 2000 technology, resulted in a total of 138 million, 100 base pair single end reads, yielding 13.8 gigabases of sequence. Forty genes were differentially expressed at a significance level of P-value < 0.05 and a fold change ≥ 2 in response to chronic, cyclic heat stress (mid-point of the last day of a 7-day cyclic heat stress of 7 hours per day), with 27 down-regulated and 13 up-regulated. Two gene networks were created from the function-based Ingenuity Pathway Analysis (IPA) of the differentially expressed genes; “Cell Signaling, Molecular Transport, Small Molecule Biochemistry” and “Endocrine System Development and Function, Small Molecule Biochemistry Cell Signaling”. Members of the MAPK signaling pathway and differentially expressed genes that are associated with MAPK-related functions were prominent in the networks. Cellular proliferation and differentiation, inflammationand stress-related signaling, and apoptosis-associated genes were down-regulated in response to heat stress. Genes responsible for inhibiting feed intake and sphingolipidrelated signaling were up-regulated. Genes involved with the regulation of inflammation, stress, thyroid hormone level, and body temperature were both up- and down-regulated. Conclusions Chronic, cyclic heat stress of broilers results in metabolic changes that can be characterized through RNA-seq analysis of the liver transcriptome. The primary affected pathways included cell signaling, molecular transport, endocrine system development and signaling, and small molecule biochemistry.
2013-09-20 | GSE51035 | GEO