ABSTRACT: Impacts of low temperature on the transcriptome of two invasive fruit fly species, Bactrocera dorsalis (Hendel) and Bactrocera correcta (Bezzi) (Diptera: Tephritidae)
Project description:<p>Gut commensal microbiota can play an integral role in shaping insect reproduction, but the mechanisms underlying this process remain largely unexplored. Here, we report that gut bacteria promote host reproduction in the oriental fruit fly Bactrocera dorsalis (Diptera: Tephritidae), an important agricultural pest. To do so, bacteria induce the degradation of the key transcription factor longitudinals lacking-like (Lolal) via the ubiquitin-proteasome system (UPS). Depletion of gut commensal bacteria impaired ovarian development and fertility. This reproductive defect could be reversed by nicotinic acid (NA) supplementation or recolonization with the potent NA provider Enterobacter hormaechei bacterium. Gut bacteria-derived NA promoted coenzyme NAD biosynthesis and mitochondrial energy production, which in turn activated the UPS. Ubiquitinome analysis further revealed that gut bacteria enhance Lolal ubiquitination and promote its degradation, preventing excessive Lolal accumulation. Lolal overabundance in gut bacteria-depleted females led to decapentaplegic (dpp) overexpression and impaired female reproduction. Conversely, Lolal knockdown led to decreased dpp expression resulting in the disruption of mature egg formation. Our results uncover a link between gut bacteria-derived metabolites and host protein homeostasis which determines host reproduction.</p>
Project description:A transcriptional and functional analysis of heat hardening in two invasive fruit fly species, Bactrocera dorsalis and Bactrocera correcta
| PRJNA486214 | ENA
Project description:A transcriptional and functional analysis of heat hardening in two invasive fruit fly species, Bactrocera dorsalis and Bactrocera correcta
| PRJNA486250 | ENA
Project description:Transcriptomic changes in the pre-blastoderm embryos of the Oriental fruit fly, Bactrocera dorsalis (Hendel) (Diptera: Tephritidae) microinjected with white locus CRISPR/Cas9 RNP complex
Project description:we used an integrated approach for the elucidation of the reproductive system and mating procedures of the olive fruit fly, Bactrocera oleae. Initially, we performed RNAseq analysis in reproductive tissues of virgin and mated insects. Comparison of the transcriptomes resulted in the identification of genes that are differentially expressed after mating.
2021-02-18 | GSE166965 | GEO
Project description:Transcriptome of Kiwifruit in Response to Bactrocera dorsalis (Hendel) Feeding
Project description:We constructed three small RNA libraries from embryos at 5, 6 and 7 post-oviposition (hpo) of Bactrocera dorsalis for deep sequencing. The data analysis revealed 147 known and 103 novel miRNAs from these libraries.
