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Molecular Characteristics and Biological Functions of Hypoxic Microenvironment in Hepatocellular Carcinoma

ABSTRACT: Molecular Characteristics and Biological Functions of Hypoxic Microenvironment in Hepatocellular Carcinoma

PROVIDER: PRJNA833336 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR18971100_1.fastq.gz	Fastqsanger.gz
	SRR18971100_2.fastq.gz	Fastqsanger.gz
	SRR18971101_1.fastq.gz	Fastqsanger.gz
	SRR18971101_2.fastq.gz	Fastqsanger.gz
	SRR18971102_1.fastq.gz	Fastqsanger.gz

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Project description:Hypoxia rapidly alters gene expression to allow cellular adaptation to challenging conditions and support tumour growth. Hypoxia also affects the chromatin structure by modifications of histones and DNA methylation. Glioblastoma (GBM) is an aggressive, deadly primary brain tumour for which there is no effective treatment. The tumour microenvironment of GBM is highly heterogeneous, with infiltration of glioma-associated microglia and macrophages (GAMs) and the presence of necrotic, hypoxic regions. The mechanisms through which hypoxia alters the tumour microenvironment and regulates functions of infiltrating immune cells remain poorly understood. Here, we show that hypoxia modulates the expression of myeloid markers in distinct ways: upregulates the monocytic marker Lgals3 expression and downregulates the microglial markers P2ry12 and Tmem119 in microglial and monocytic GAMs in vitro and in vivo, as shown using human and mouse GBM single-cell transcriptomics datasets. The genome-wide hypoxia-dependent transcriptomic changes in microglial cells were determined in microglia-glioma co-cultures. Numerous GAM subtype markers were dysregulated in response to hypoxic stress due to associated changes in chromatin accessibility, as determined using ATACseq. While hypoxia alone drives a decrease of the overall chromatin accessibility at gene promoters, the exposure to glioma cells under hypoxic conditions leads to both increases and decreases of chromatin accessibility at promoter regions in microglial cells. Hypoxia downregulates the chromatin accessibility at the regions enriched in motifs of transcription factors regarded as master regulators of microglial cell identity and function, including SPI1 or IRF8. Moreover, changes in histone acetylation are responsible for Lgals3 upregulation and lipid accumulation in GAMs in hypoxic conditions. Overall, our data highlights the importance of hypoxic stress as a strong intratumoral regulator of myeloid cell functions, which adds complexity to the characterisation of particular GAMs subpopulations.

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Molecular Characteristics and Biological Functions of Hypoxic Microenvironment in Hepatocellular Carcinoma

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