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Whole-transcriptome analysis of hematopoietic progenitors in the cranial dura, femur, and skull

ABSTRACT: Whole-transcriptome analysis of hematopoietic progenitors in the cranial dura, femur, and skull

PROVIDER: PRJNA833880 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR19023614_1.fastq.gz	Fastqsanger.gz
	SRR19023614_2.fastq.gz	Fastqsanger.gz
	SRR19023615_1.fastq.gz	Fastqsanger.gz
	SRR19023615_2.fastq.gz	Fastqsanger.gz
	SRR19023616_1.fastq.gz	Fastqsanger.gz

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Whole-transcriptome analysis of hematopoietic progenitors in the cranial dura, femur, and skull

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Project description:The dura mater hosts a rich population of B cell progenitors, yet its capacity to sustain B cell development during systemic lymphopenia remains unclear. Using Trypanosoma brucei infection, which induces peripheral B cell depletion, we demonstrate that the murine dura mater maintains intact B cell lymphopoiesis independently of bone marrow and spleen. We performed a comparative single cell transcriptomic profiling of the calvaria bone marrow, femur bone marrow, and dura mater from naive and Trypanosoma brucei-infected female mice. The sequencing rendered high quality data from the calvaria bone marrow and the dura mater, but the femur bone marrow failed to generated reliable data and was thus excluded from downstream analyses. This data is single-cell RNA sequencing using Parse Biosciences Evercode Whole Transcriptome combinatorial barcoding

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Lymphoid structures in the skull bone marrow control homeostatic and anti-tumor immune responses in the CNS [dura/skull/sternum]

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Transcriptome analysis of the cranial dura of neonatal C57 BL/6 mice

Project description:To investigate the possible changes of meningeal gene expression during neonatal stage, we performed gene expression profiling analysis using data obtained from bulk RNA-seq of the cranial dura of C57 BL/6 mice on postnatal day1,day7, and day14.

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Lymphoid structures in the skull bone marrow control homeostatic and anti-tumor immune responses in the CNS [dura/skull/sternum]

Project description:Lymphoid structures in the skull bone marrow control homeostatic and anti-tumor immune responses in the CNS [dura/skull/sternum]

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B cells and their progenitors reside in homeostatic meninges

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