Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Chronic chemogenetic activation of the superior colliculus in glaucomatous mice: local and retrograde molecular signature

ABSTRACT: Chronic chemogenetic activation of the superior colliculus in glaucomatous mice: local and retrograde molecular signature

PROVIDER: PRJNA834042 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR19032800.fastq.gz	Fastqsanger.gz
	SRR19032900.fastq.gz	Fastqsanger.gz
	SRR19033000.fastq.gz	Fastqsanger.gz
	SRR19032801.fastq.gz	Fastqsanger.gz
	SRR19032901.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 287

Similar Datasets

Chronic chemogenetic activation of the superior colliculus in glaucomatous mice: local and retrograde molecular signature

Project description:One important facet of the glaucoma pathology is axonal damage, which ultimately disrupts the connection between the retina and its postsynaptic targets. The concurrent loss of retrograde support interferes with the functionality and survival of the retinal ganglion cells (RGCs). Con-versely, previous research has shown that stimulation of neuronal activity in a primary retinal target area—i.e., the superior colliculus— promotes RGC survival in an acute mouse model of glaucoma. To build further on this observation, we applied repeated chemogenetics in the superior colliculus of a more chronic model of glaucoma—i.e., the microbead occlusion model—and per-formed bulk RNA sequencing on collicular lysates and isolated RGCs. Our study revealed that chronic target stimulation upon glaucomatous injury phenocopies the a priori expected molecular response: growth factors were pinpointed as essential transcriptional regulators both in the locally stimulated tissue and in distant, unstimulated RGCs. Strikingly, and although the RGC tran-scriptome revealed an enrichment of pro-survival signaling pathways, functional rescue of injured RGCs was not achieved.

2022-06-15 | GSE202012 | GEO

Chronic Chemogenetic Activation of the Superior Colliculus in Glaucomatous Mice: Local and Retrograde Molecular Signature.

Project description:One important facet of glaucoma pathophysiology is axonal damage, which ultimately disrupts the connection between the retina and its postsynaptic brain targets. The concurrent loss of retrograde support interferes with the functionality and survival of the retinal ganglion cells (RGCs). Previous research has shown that stimulation of neuronal activity in a primary retinal target area-i.e., the superior colliculus-promotes RGC survival in an acute mouse model of glaucoma. To build further on this observation, we applied repeated chemogenetics in the superior colliculus of a more chronic murine glaucoma model-i.e., the microbead occlusion model-and performed bulk RNA sequencing on collicular lysates and isolated RGCs. Our study revealed that chronic target stimulation upon glaucomatous injury phenocopies the a priori expected molecular response: growth factors were pinpointed as essential transcriptional regulators both in the locally stimulated tissue and in distant, unstimulated RGCs. Strikingly, and although the RGC transcriptome revealed a partial reversal of the glaucomatous signature and an enrichment of pro-survival signaling pathways, functional rescue of injured RGCs was not achieved. By postulating various explanations for the lack of RGC neuroprotection, we aim to warrant researchers and drug developers for the complexity of chronic neuromodulation and growth factor signaling.

| S-EPMC9179903 | biostudies-literature

Effects of eye opening on gene expression in the superficial layers of the rat superior colliculus

Project description:We performed RNA-seq on six samples of P20 Sprague Dawley rat superior colliculus, three control rats that underwent eye opening at P14 until P20 and three deprived rats which had their eyes glued closed from P14 until P20. Each sample was created from two pooled colliculi. RNA-seq of the superior colliculus with and without long term light deprivation.

2015-09-09 | E-GEOD-72787 | biostudies-arrayexpress

Modular organization of the motor space in the mouse superior colliculus

Project description:In order to characterize the genetic diversity within the Stratum Griseum Intermediale (SGI), we screened gene expression by extracting RNA from sensory and motor layers of the Superior Colliculus (SC) at post-natal day (P)7.

2019-07-31 | GSE135082 | GEO

Mapping Visual Functions onto Molecular Cell Types in the Mouse Superior Colliculus

Project description:To facilitate investigation of function, connectivity, and development of superficial superior colliculus (sSC), we identified 28 neuron subtypes and subtype-enriched marker genes from tens of thousands of adult mouse sSC neurons

2023-04-21 | GSE223155 | GEO

Multipotent Progenitors Instruct Ontogeny of the Superior Colliculus

Project description:Multipotent Progenitors Instruct Ontogeny of the Superior Colliculus

| PRJNA1026945 | ENA

Multipotent Progenitors Instruct Ontogeny of the Superior Colliculus – Pten

Project description:Multipotent Progenitors Instruct Ontogeny of the Superior Colliculus – Pten

| PRJNA1026955 | ENA

Effects of eye opening on gene expression in the superficial layers of the rat superior colliculus

2015-09-09 | GSE72787 | GEO

Multipotent Progenitors Instruct Ontogeny of the Superior Colliculus – Pten

Project description:The superior colliculus (SC) in the mammalian midbrain is essential for multisensory integration and is composed of a rich diversity of excitatory and inhibitory neurons and glia. The developmental principles directing the generation of SC cell-type diversity are however not understood. Here we pursued systematic cell lineage tracing in silico and in vivo, preserving full spatial information, using genetic MADM (Mosaic Analysis with Double Markers)-based clonal analysis with single-cell sequencing (MADM-CloneSeq). The analysis of clonally-related cell lineages revealed that radial glial progenitor (RGP) cells in SC are exceptionally multipotent. Individual resident RGPs have the capacity to produce all excitatory and inhibitory SC neuron types, even at the stage of terminal division. While individual clonal units show no pre-defined cellular composition, the establishment of appropriate relative proportions of distinct neuronal types occurs in a PTEN-dependent manner. Collectively, our findings provide an inaugural framework at single RGP/cell level of the mammalian SC ontogeny

2023-11-03 | GSE245104 | GEO

Multipotent Progenitors Instruct Ontogeny of the Superior Colliculus – MADM-CloneSeq E10

Project description:Multipotent Progenitors Instruct Ontogeny of the Superior Colliculus – MADM-CloneSeq E10

| PRJNA1026948 | ENA