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METTL3 inhibits inflammation of retinal pigment epithelium cells by regulating NR2F1 in an m6A-dependent manner [meRIP-seq]

ABSTRACT: METTL3 inhibits inflammation of retinal pigment epithelium cells by regulating NR2F1 in an m6A-dependent manner [meRIP-seq]

PROVIDER: PRJNA834054 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR19033203_1.fastq.gz	Fastqsanger.gz
	SRR19033203_2.fastq.gz	Fastqsanger.gz
	SRR19033204_1.fastq.gz	Fastqsanger.gz
	SRR19033204_2.fastq.gz	Fastqsanger.gz
	SRR19033205_1.fastq.gz	Fastqsanger.gz

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Similar Datasets

METTL3 inhibits inflammation of retinal pigment epithelium cells by regulating NR2F1 in an m6A-dependent manner

Project description:METTL3 inhibits inflammation of retinal pigment epithelium cells by regulating NR2F1 in an m6A-dependent manner

| PRJNA834049 | ENA

METTL3 inhibits inflammation of retinal pigment epithelium cells by regulating NR2F1 in an m6A-dependent manner [RNA-seq]

Project description:METTL3 inhibits inflammation of retinal pigment epithelium cells by regulating NR2F1 in an m6A-dependent manner [RNA-seq]

| PRJNA834053 | ENA

METTL3 inhibits inflammation of retinal pigment epithelium cells by regulating NR2F1 in an m6A-dependent manner [meRIP-seq]

Project description:To investigate the fuction of METTL3 in autoimmune uveitis, we established ARPE-19 cell lines in which target gene has been knocked down by shRNA.

2022-07-03 | GSE202016 | GEO

MeRIP-seq to explore the targets of METTL3 in pancreatic cancer

Project description:We performed MeRIP-seq of METTL3-knockdown (KD) MIAPaCa-2 and control cells to detect METTL3-methylated genes and extracted genes whose methylation was lost in METTL3-KD cells compared with control cells. Next, we performed mRNA expression analysis using dates of input samples at MeRIP-seq. Genes whose methylation peaks were abolished by METTL3-KD and whose expression changes were observed were analyzed as candidates for METTL3-regulated genes.

2021-12-13 | GSE190078 | GEO

METTL3 Facilitates Hypertrophic Scar Fibrosis by Promoting pri-miRNA-31 Maturation and ZBTB20 Silencing in m6A-dependent Manner [MeRIP-Seq]

Project description:To clarify the role of METTL3-mediated m6A modification in hypertrophic scars (HS), we conditionally knocked out the m6A methyltransferase METTL3 in HS-derived dermal fibroblasts (HSFBs). We then performed MeRIP-seq analysis on HSFBs.

2024-08-30 | GSE246978 | GEO

METTL3 inhibits inflammation of retinal pigment epithelium cells by regulating NR2F1 in an m6A-dependent manner [RNA-seq]

Project description:To investigate the fuction of METTL3 in autoimmune uveitis, we established ARPE-19 cell lines in which target gene has been knocked down by shRNA. We then performed gene expression profiling analysis using data obtained from RNA-seq between sh-Control groups and sh-METTL3 groups.

2022-07-03 | GSE202017 | GEO

MeRIP-sequencing data from H1975 cells with NC or METTL3-sh

Project description:We use MeRIP-sequencing to evaluate the effect of METTL3 knockdown on the gene changes in H1975 cells.

2020-12-07 | GSE147171 | GEO

MeRIP-Seq (m6A-Seq) identifies the mRNA m6A modification target in negative control and METTL3 knockdwon HULEC-5a

Project description:To identify the molecular mechanism by which METTL3 regulates endothelial barrier function, we performed RNA-seq and MeRIP-seq in HULEC-5a cells with stable METTL3 knockdown and control cells. The RNA-seq results revealed that 437 transcripts were significantly downregulated (fold change <0.5) after METTL3 knockdown. The MeRIP-seq results revealed that the m6A peaks in 1011 transcripts were decreased in abundance (fold change >1.2). Intriguingly, 55 transcripts overlapped in the RNA-seq and MeRIP-seq data

2022-05-20 | GSE200648 | GEO

MeRIP-seq to identify putative Mettl3 targets in the nephron progenitor cells

Project description:Mettl3 is an mRNA methyl transferase enzyme. It catalzyes the methylation of the most common mRNA biochemical modification N6-methyl adenosine (m6A). The role of Mettl3/m6A pathway is unknown in the developing kidney. Through genetic and pharmaceutical approaches we found that Mettl3 is required for the differentiation of nephron progenitor cells. nephron progenitors. Genetic deletion of Mettl3 in the nephron progenitor cells blocks their differentiation. We sought to identify the mechanism of Mettl3 activity. We performed RNA-sequencing and RNA-m6A pull down assay followed by next-generation sequencing (MeRIP-seq) on wild type and Mettl3 deleted kidneys to determine the mRNA targets of Mettl3.

2025-04-14 | GSE294483 | GEO

METTL3 mediated m6A mRNA methylation regulates neutrophil activation through targeting TLR4 signaling (meRIP-Seq)

Project description:To investigate the effect of METTL3 function in the regulation of neutrophil activation, we established neutrophil METTL3 knock out mice by crossing METTL3flox/flox mice with Lyzm-cre mice. We then performed gene expression profiling analysis using data obtained from MeRIP-seq of isolated neutrophil from METTL3f/f and METTL3f/f Lyzm-Cre mice .

2023-03-31 | GSE225139 | GEO

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