Project description:We performed single-cell RNA sequencing of lung cancer patients receiving immune checkpoint inhibitors. Tumor signatures and immune cell profiles associated with ICI response were identified in lung cancer.
Project description:Microarray gene expression of peripheral blood of the prostate cancer patients receiving localized external beam radiation therapy (EBRT) Assay processed at EBRT time points, baseline, midpoint (days 19-21) and endpoint (days 38-42)
Project description:The etiology behind cancer-related fatigue (CRF) is currently unknown. The physiological mechanisms of CRF are based on limited evidence that genetic factors, energy expenditure, metabolism, aerobic capacity, and the individual's immune response to inflammation are responsible for the experience of CRF. Gene expression profiling using microarray analysis from white blood cells of men with non-metastatic prostate cancer shows significant, differential expression of 463 probesets during localized external beam radiation therapy (EBRT). Pathway analysis shows a central role of SNCA (alpha-synuclein gene) among these differentially expressed probesets. Significant expression of SNCA was confirmed by qPCR (p<.001) and ELISA (p<.001) over time during EBRT. A significant correlation was noted between averaged fatigue scores and delta CT values of SNCA expression using confirmatory qPCR over time during EBRT (R=-.90, p=.006). Development of fatigue experienced by these men during EBRT may be mediated by SNCA expression. Pathways related to alpha-synuclein may serve as useful biomarkers to understand the mechanisms behind the development of fatigue. A longitudinal design exploring the association between changes in gene expression and fatigue symptoms of men with non-metastatic prostate cancer receiving external beam radiation therapy. Blood samples were collected from ten subjects at 7 timepoints for microarray analysis: baseline (before EBRT); days 1, 7, 14, 21, 42 of EBRT; and 30 days post-EBRT. Baseline data obtained from subjects were compared to data obtained from age-, race-, and gender-matched healthy controls.
Project description:Human immunodeficiency virus is a cause of progressive infection leading to immune dysfunction. Although antiretroviral treatment can normalize the functional state of immune cells by reducing viral load, for some people the immune efficacy of antiretroviral therapy (ART) is insufficient. The aim of this study was to investigate the transcriptomic changes in peripheral blood mononuclear cells (PBMC) of patients receiving ART. To evaluate the changes in PBMC, we conducted a clinical trial involving HIV-positive patients on ART. PBMC were collected before starting ART and after 24 weeks of treatment with ART along with estimation of viral load and CD4+ and CD8+ T-lymphocytes count. To evaluate the transcriptional profiles, we used RNA sequencing of isolated PBMC. The results showed two-fold differential expression of 70 genes in PBMC induced by ART and 56 genes prediction an increase in CD4+ lymphocytes after therapy. This information can be helpful for understanding molecular mechanisms of host response to ART.
Project description:Tuberculosis-associated Immune Reconstitution Inflammatory Syndrome (TB-IRIS) is a common complication in HIV-TB co-infected patients receiving combined antiretroviral therapy (cART). While monocytes/macrophages play major roles in both HIV- and TB-infection individually, a putative contribution of monocytes to the development of TB-IRIS remains unexamined. We performed a genome-wide array analysis on MOs purified from peripheral blood mononuclear cells (PBMCs) obtained before initiation of combined antiretroviral therapy (cART) to verify whether the transcriptome of MOs was already significantly modulated (even before receiving cART) in HIV+/TB+ patients who later developed TB-IRIS compared to control HIV+/TB+ patients who did not develop the complication . The subjects under study included a subset of 18 TB-IRIS patients and controls matched for age, gender and CD4 count.
Project description:immunotherapy offers a better prognosis for pancreatic cancer patients. As a direct extension of this work, various new therapy methods that are under exploration and clinical trials could be assessed or evaluated using the newly developed mathematical prognosis model.