Project description:We found two DNA binding proteins, CRP and PepA, associate at the membrane. This study was performed to determine if their interaction affects transcription activity..
Project description:We found two DNA binding proteins, CRP and PepA, associate at the membrane. This study was performed to determine if their interaction affects DNA binding.
Project description:Within this study, we aimed to investigate how enviornmental nitrate effects the transcription of the Vibrio cholerae genome. Here we performed RNASeq using static cultures of WT, dCrp, dHapR, & dhapR/Crp.
Project description:Transcriptional profiling of an El Tor biotype crp mutant The virulent V. cholerae El Tor Ogawa strain C7258 (Peru isolate 1991) and an isogenic deletion mutant (WL7258) lacking DNA sequences encoding the cAMP receptor protein were grown in LB medium to optical density at 600 nm of 1.5. The cultures were chilled in ice, cells quickly collected by centrifugation and total RNA imediately extracted. RNAwas extracted and purified using the Trizol plus RNA purification system (Invitrogen) followed RNEasy miniElute cleanup (Qiagen). RNA samples were conserved at - 80 C and used within a week. Keywords: Genetic modification
Project description:The experiment contains ChIP-seq data for Vibrio cholerae strain N16961. The strain was grown at 37 degrees in M9 minimal medium and crosslinked with 1 % (v/v) formaldehyde. After sonication, to break open cells and fragment DNA, immunoprecipitations were done using no antibodies (mock immunoprecipitations) or antibodies against CRP or the RNA polymerase sigma 70 subunit. Libraries were prepared using DNA remaining after immunoprecipitation.
Project description:Transcriptional profiling of an El Tor biotype crp mutant The virulent V. cholerae El Tor Ogawa strain C7258 (Peru isolate 1991) and an isogenic deletion mutant (WL7258) lacking DNA sequences encoding the cAMP receptor protein were grown in LB medium to optical density at 600 nm of 1.5. The cultures were chilled in ice, cells quickly collected by centrifugation and total RNA imediately extracted. RNAwas extracted and purified using the Trizol plus RNA purification system (Invitrogen) followed RNEasy miniElute cleanup (Qiagen). RNA samples were conserved at - 80 C and used within a week. 4 replicates
Project description:RNA-seq was performed to compare the transcriptomes of Vibrio cholerae C6706 strains expressing V5-tagged wild-type CRP (CRP-V5) or the acetylation-mimicking mutant K52Q-V5 during growth in M9 minimal medium supplemented with 0.8% maltose or 0.8% trehalose as the sole carbon source. Overnight cultures grown in LB were washed and resuspended in the respective M9 media, then cultivated for approximately 3 hours to mid-exponential phase. Total RNA was extracted and subjected to stranded total RNA sequencing with ribosomal RNA depletion. This dataset complements ChIP-seq and RNA-seq data previously deposited under GSE310654 by examining CRP-dependent transcriptional responses under carbon source-specific conditions.
