Project description:Live single-cell RNA-sequencing data from ALS donors only. Single-cell RNA-sequencing data on CD45+ cells dissociated from autopsy tissue and surgical resections from ALS donors across a different series of brain regions.
Project description:This dataset includes DIA-PASEF-based plasma proteomics and single-cell proteomics of peripheral T and B cells from Amyotrophic Lateral Sclerosis (ALS) patients and healthy controls, enabling analysis of disease progression, identification of differentially expressed proteins, and investigation of immune-axonal regulatory mechanisms relevant to ALS pathogenesis.
Project description:The mutation in the C9orf72 gene with a hexanucleotide repeat has been reported multiple times to be the most common genetic cause of FTD and ALS (Frontotemporal Dementia and Amyotrophic Lateral Sclerosis), both of which are devastating neurodegenerative diseases having no cures currently. Our lab previously created fruit fly models expressing 36(C4G2) repeats, these are highly toxic to adult neurons of fruit flies. This is one of the most commonly used fly models of disease. Like many neurodegenerative diseases, FTD and ALS display selective neuronal vulnerability: only some neuronal populations succumb to disease, even though the toxic species are ubiquitously expressed. Our lab proposes to identify which neuronal populations are selectively depleted in response to the expression of the repeats and analyse which pathways are activated in vulnerable and resistant neuronal populations using our fly model of disease. This is done by scRNA sequencing across multiple time points, tracking disease development. The workflow was first having the flies ready and their brains being dissected. The brains were then dissociated by collagenase and dispase, and the cell suspensions were passed through a 10um cell strainer. The single-cell suspensions were checked for viability and the single-cell libraries were prepared with 10X Chromium 3' platform.
Project description:Transcriptomic analysis of postmortem cervical spinal cord samples from 8 people with ALS (6 sporadic and 2 familial cases) and 4 age-matched non-neurological controls using snRNA-seq
Project description:Single-cell RNA-sequencing was conducted on heterogeneous human prostates FACS sorted for viability from organ donors (with no known prostatic diseases) on the 10x Genomics platform.
Project description:Blood from two donors was incubated for timepoints up to 24 hours in the presence and absence of live meningococci. The donors are labelled NP and SW. 'SWB' refers to blood that was incubated with meningococci; 'Unstim' refers to control blood incubated in the absence of meningococci. Duplicate arrays were prepared for the majority of timepoints ('a' and 'b'), except where the array of of poor quality and not used in the analysis.