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Transcriptomic profiling of breast cancer cells on hydrogels with different stiffness [array]

ABSTRACT: Transcriptomic profiling of breast cancer cells on hydrogels with different stiffness [array]

PROVIDER: PRJNA847897 | ENA |

REPOSITORIES: ENA

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Transcriptomic profiling of breast cancer cells on hydrogels with different stiffness [array]

Project description:Remodeling and increased stiffness of the extracellular matrix is a hallmark of solid cancers and contributes to tumor progression through increased proliferation, survival and migration. Mechanotransduction, the process in which cells sense and transduce mechanical signals, can result in transcriptional changes that in turn alter cellular behavior. In vitro culturing of MCF10ACA1a (CA1a) breast cancer cells on polyacrylamide-based hydrogels of variable stiffness revealed drastic changes in morphology, indicating a stiffness dependent induction of a malignant phenotype. We used DNA microarray to characterize the transcriptome of CA1a cells on low (400 Pa) and high (5000 Pa) stiffness, corresponding to the stiffness of normal breast and breast cancer, respectively.

2023-03-13 | GSE205817 | GEO

Transcriptomic profiling of breast cancer cells on hydrogels with different stiffness [sequencing]

Project description:Remodeling and increased stiffness of the extracellular matrix is a hallmark of solid cancers and contributes to tumor progression through increased proliferation, survival and migration. Mechanotransduction, the process in which cells sense and transduce mechanical signals, can result in transcriptional changes that in turn alter cellular behavior. In vitro culturing of MCF10ACA1a (CA1a) breast cancer cells on polyacrylamide-based hydrogels of variable stiffness revealed drastic changes in morphology, indicating a stiffness dependent induction of a malignant phenotype. We used two different techniques, DNA microarray and RNA sequencing, to characterize the transcriptome of CA1a cells on low (500 Pa) and high (8000 Pa) stiffness, corresponding to the stiffness of normal breast and breast cancer, respectively.

2023-03-13 | GSE205816 | GEO

Transcriptomic profiling of breast cancer cells on hydrogels with different stiffness

Project description:Transcriptomic profiling of breast cancer cells on hydrogels with different stiffness

| PRJNA847893 | ENA

Transcriptomic profiling of breast cancer cells on hydrogels with different stiffness [sequencing]

Project description:Transcriptomic profiling of breast cancer cells on hydrogels with different stiffness [sequencing]

| PRJNA847898 | ENA

RNA-seq of 4T1 mouse breast cancer cells cultured on soft vs stiff polyacrylamide hydrogels for 24 hours

Project description:The tumour microenvironment is a critical element involved in tumour progression and responsiveness to therapies. Using functionalized tunable stiffness hydrogel, mimicking the mechanical properties of healthy and tumour tissues, we explore how the stiffness of the microenvironment can influence cancer cells by generating RNA-seq transcriptional profiles of 4T1 mouse breast cancer cells cultured on soft vs stiff polyacrylamide hydrogels for 24 hours.

2024-12-30 | E-MTAB-11487 | biostudies-arrayexpress

Gene expression profiling of neural stem cells in 3D hydrogels with different stiffness

Project description:Gene expression profiling of neural stem cells in 3D hydrogels with different stiffness

| PRJNA795362 | ENA

Gene expression profiling of neural stem cells in 3D hydrogels with different stiffness

Project description:We report a transcriptome sequencing to identify 3D hydrogel stiffness-dependent genes

2022-01-14 | GSE193194 | GEO

Extracellular stiffness regulates site-specific lung development [AWO]

Project description:Extracellular matrix (ECM) stiffness plays a crucial role in regulating cell fate and maturation, but its influence on lung development is limited known. Here we utilized stiffness-tunable gelatin methacryloyl (GelMA) hydrogels to investigate how ECM stiffness influences site-specific lung development in a stem cell-derived lung organoid model. We found increased stiffness promoted NKX2-1+ lung progenitor cells (LPCs) generation. In airway organoids (hAWOs), stiff hydrogels directed proximal airway differentiation enriched with goblet, ciliated, and basal cells; whereas the decreased stiffness favored emergence of secretory cells in the proximal-distal transition zone and distal airway. In alveolar organoids (hALOs), increased stiffness enhanced AT2 and AT1 cells transition. Moreover, infection assays with Omicron BA.1.1 and Delta variants recapitulated the proximal-to-distal tropism of SARS-CoV-2 in the lung. Transcriptomic sequencing revealed ECM stiffness regulates lung development via Hippo, TGF-β, HIF and Wnt pathways. These findings advance mechanism understanding of ECM stiffness on lung development and provide a novel mechanical regulation for generating site-specific lung organoids.

2026-06-02 | GSE293170 | GEO

Extracellular stiffness regulates site-specific lung development [ALO]

2026-06-02 | GSE292932 | GEO

Study of gene expression in mouse primary hepatocytes cultured in hydrogels with different stiffnesses in wild-type and YAP/TAZ knockout.

Project description:Liver stiffness is extensively utilized as a crucial indicator in the diagnosis and monitoring of MASLD, the contribution of increased stiffness on impaired liver functions and its molecular mechanisms remains poorly understood. Wild-type or Yap/Taz knock-out hepatocytes were cultured on soft (1 kPa) and stiff (20 kPa) hydrogels to embody healthy and fibrotic livers and subjected to metabolomic and transcriptomic analyses.

2026-05-15 | GSE274047 | GEO

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