Project description:The keratinocyte cell line HaCaT was cultured for three days (proliferation) or for ten days (differentiation). RNA from cells at day3 was compared to RNA from cells at day10. The microarray hybridizations were performed in dye-swap procedure : RNA from cells at day3 was labeled with Cy3 (GSM4674, GSM4675, GSM4682, GSM4683) and then with cy5 (GSM4680, GSM4681). Keywords = cell density differentiation program in human keratinocytes Keywords: repeat sample
Project description:The keratinocyte cell line HaCaT was cultured for three days (proliferation) or for ten days (differentiation). RNA from cells at day3 was compared to RNA from cells at day10. The microarray hybridizations were performed in dye-swap procedure : RNA from cells at day3 was labeled with Cy3 (GSM4674, GSM4675, GSM4682, GSM4683) and then with cy5 (GSM4680, GSM4681). Keywords = cell density differentiation program in human keratinocytes
Project description:Human HaCaT keratinocytes were cultured to confluence (day10). Differentiated confluent cells were irradiated at 0.5 Gy or 2 Gy. RNA from irradiated cells was compared to RNA from non-irradiated reference cells in a dye-swap hybridization procedure 24 h after irradiation. Keywords = human keratinocytes, gamma irrdiation
Project description:Human HaCaT keratinocytes were cultured to confluence (day10). Differentiated confluent cells were irradiated at 0.5 Gy or 2 Gy. RNA from irradiated cells was compared to RNA from non-irradiated reference cells in a dye swap hybridization procedure 3 h after irradiation. Keywords = Human keratinocytes, gamma irradiation
Project description:Huamn HaCaT keratinocytes were cultured to confluence (day10). Differentiated confluent cells were irradiated at 0.5 Gy or 2 Gy. RNA from irradiated cells was compared to RNA from non-irradiated reference cells in a dye-swap hybridization procedure 3 h after irradiation. Keywords = Human keratinocytes, gamma irradiation Keywords: dose response
Project description:Human HaCaT keratinocytes were cultured to confluence (day10). Differentiated confluent cells were irradiated at 0.5 Gy or 2 Gy. RNA from irradiated cells was compared to RNA from non-irradiated reference cells in a dye swap hybridization procedure 3 h after irradiation. Keywords = Human keratinocytes, gamma irradiation Keywords: dose response
Project description:Human HaCaT keratinocytes were cultured to confluence (day10). Differentiated confluent cells were irradiated at 0.5 Gy or 2 Gy. RNA from irradiated cells was compared to RNA from non-irradiated reference cells in a dye-swap hybridization procedure 24 h after irradiation. Keywords = human keratinocytes, gamma irrdiation Keywords: dose response
Project description:Huamn HaCaT keratinocytes were cultured to confluence (day10). Differentiated confluent cells were irradiated at 0.5 Gy or 2 Gy. RNA from irradiated cells was compared to RNA from non-irradiated reference cells in a dye-swap hybridization procedure 3 h after irradiation. Keywords = Human keratinocytes, gamma irradiation Keywords: dose response
Project description:To investigate the regulatory effects of the ECM@SF-AGS-CS-LA hydrogel (HC), exosomes (HB), and their combination (HA) on the metabolic profiles of wound tissues in diabetic mice, HaCaT keratinocytes were employed as an in vitro cellular model. Untargeted metabolomics profiling was performed on HaCaT cells subjected to the respective treatments. Five pairwise comparisons were conducted: HC versus HD (diabetic control), HB versus HD, HA versus HD, HA versus HC, and HA versus HB. KEGG pathway enrichment analysis was applied to identify biologically relevant pathways significantly associated with differentially abundant metabolites (P < 0.05). This analysis revealed 8, 28, 30, 30, and 97 significantly enriched pathways in the HC vs HD, HB vs HD, HA vs HD, HA vs HC, and HA vs HB comparisons, respectively.