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ABSTRACT: Donkey oral bacteria

PROVIDER: PRJNA851537 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR19776875_1.fastq.gz	Fastqsanger.gz
	SRR19776875_2.fastq.gz	Fastqsanger.gz
	SRR19776876_1.fastq.gz	Fastqsanger.gz
	SRR19776876_2.fastq.gz	Fastqsanger.gz
	SRR19776877_1.fastq.gz	Fastqsanger.gz

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Donkey milk fermentation by Lactococcus lactis subsp. cremoris and Lactobacillus rhamnosus affects the antiviral and antibacterial milk properties

Project description:Background: milk is considered an important source of bioactive peptides, which can be produced by endogenous or starter bacteria, such as lactic acid bacteria, that are considered effective and safe producers of food-grade bioactive peptides. Among the various types of milk, donkey milk has been gaining more and more attention for its nutraceutical properties. Methods: Lactobacillus rhamnosus 17D10 and Lactococcus lactis subsp. cremoris 40FEL3 were selected for their ability to produce peptides from donkey milk. The endogenous peptides and those obtained after bacterial fermentation were assayed for their antioxidant, antibacterial and antiviral activities. The peptide mixtures were characterized by means of LC-MS/MS, and then analyzed in silico using the Milk Bioactive Peptide DataBase. Results: the peptides produced by the two selected bacteria enhanced the antioxidant activity and reduced E. coli growth. Only the peptides produced by L. rhamnosus 17D10 were able to reduce S. aureus growth. All the peptide mixtures were able to inhibit the replication of HSV-1 by more than 50%. Seventeen peptides were found to have 60% sequence similarity with already known bioactive peptides. Conclusion: a lactic acid bacterium fermentation process is able to enhance the value of donkey milk through bioactivities that are important for human health.

2021-09-07 | PXD027765 | Pride

Project description:16S rRNA of Donkey Skin Bacteria

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Single-Cell RNA Sequencing unveils dynamic transcriptional profiles during the process of donkey spermatogenesis and maturation

Project description:With the increasing demand for donkey production, there has been a growing focus on the breeding of donkeys. However, our current understanding of the mechanisms underlying spermatogenesis and maturation in donkeys during reproduction remains limited.In this study, we constructed a single-cell RNA dataset to study the single-cell landscape of donkey spermatogenesis and maturation. This method allows us to analyze the cell composition in testicular and epididymal tissue, providing insights into the changes that occur during donkey spermatogenesis and maturation. In addition, different gene expression signatures associated with various spermatogenic cell types were found

2024-12-30 | GSE282737 | GEO

Analysis of miRNA expression in human Oral epithelial cells induced by S. gordonii

Project description:The mechanisms through which oral commensal bacteria mitigates uncontrolled inflammatory responses in the oral mucosa remain unknown. Here we evaluated the ability of S. gordonii to stimulate the expression of miRNAs in oral epithelial cells with potential to target chemokine expression. The human oral epithelial cell line (OKF6) was exposed to different MOIs of S. gordonii for 24h and expression analysis of miRNAs performed using the Affymetrix platform.

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Synergistic Exacerbation of Oral Streptococci-induced pneumonia due to Prevotella intermedia

Project description:Anaerobic bacteria in the oral cavity can cause respiratory infections. However, their precise mechanisms of action remain elusive. Unexpectedly, bacterial flora analysis using 16s rRNA revealed ‘hidden’ mixed infections of anaerobic bacteria and commensal oral Streptococcus species in community-acquired pneumonia. The purpose of this study is to elucidate the mechanisms by which Prevotella intermedia exacerbates oral streptococcal pneumonia.

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The composition of the bacteria found in liver, spleen, oral cavity and fecal samples of mice subjected to an experimental periodontitis model

Project description:Periodontitis patients often develop bacteremia, but there has been little evidence showing that oral bacteria translocate into other organs. We found that bacterial colony formation occurs in a culture of liver and spleen cells of periodontitis-induced mice, and the bacterial species detected in the liver and spleen were found in the oral cavity as well, but not in fecal samples, indicating systemic dissemination of oral bacteria during the breakdown of the oral barrier.

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Comparative Proteomic Analysis of Extracellular Vesicles from Donkey Colostrum and Mature Milk

Project description:Donkey milk (DM) has been considered a valuable alternative to human and bovine coun-terparts as well as to infant formulas. Milk extracellular vesicles (EVs) have been proposed to influence key biological processes. The purpose of this study is to provide a compre-hensive characterization of the protein composition of extracellular vesicles (EVs) by ex-tending quantitative proteomic comparisons to EVs derived from donkey colostrum (DC) and mature donkey milk (MDM). The EVs were isolated from DC and MDM samples, characterized, and subjected to proteomic analysis using the tandem mass tag-based quantitative approach

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Zearalenone exposure enhanced the expression of tumorigenesis genes in donkey granulosa cells via the PTEN/PI3K/AKT signaling pathway

Project description:We investigated the biological effects of ZEA exposure on donkey granulosa cells by using RNA-seq analysis. ZEA at 10 and 30 μM were administered to granulosa cells within 72 hours of in vitro culture. ZEA at 10 μM significantly altered the tumorigenesis associated genes in donkey granulosa cells. Exposure to 10 and 30 μM ZEA treatment significantly reduced mRNA expression of PTEN, TGFβ, ATM, and CDK2 genes, particularly, the ZEA treatment significantly increased the expression of PI3K and AKT genes. Furthermore, immunofluorescence, RT-qPCR, and Western blot analysis verified the gene expression of ZEA-exposed granulosa cells. Collectively, these results demonstrated the deleterious effect of ZEA exposure on the induction of ovarian cancer related genes via the PTEN/PI3K/AKT signaling pathway in donkey granulosa cells in vitro.

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