Project description:To clarify the mechanism of Efp/TRIM25 in breast cancer, breast cancer cells (MDA-MB-231 and TNBC-PDC) were transfected with siRNAs targeting Efp (siEfp_1 and siEfp_3) and control siRNA targeting firefly luciferase (siCont). We used microarrays to detail the global programme of Efp-mediated gene expression and identified up- and down-regulated genes.
Project description:To examine the role of Efp in endometrial cancer, patient-derived cells were treated with siRNA targeting Efp (siEfp) or control siRNA (siControl). siRNA-mediated Efp silencing resulted in suppressed spheroid proliferation and altered gene expression profile, featuring downregulation of genes related to cell cycle and inflammation/immune responses. This study provides an insight into alternative endometrial cancer therapeutic strategies targeting Efp.
Project description:To examine the role of Efp in endometrial cancer cells, Ishikawa and HEC-1A cells were treated with siRNA targeting Efp (siEfp) or control siRNA (siControl). Microarray analysis showed that Efp knockdown represses NF-κB-signaling pathway-associated genes.