Project description:Comparison of gene expression data from thalidomide modulated- and unmodulated-MSCs from patients with idiopathic thrombocytopenic purpura (ITP).
Project description:Comparison of gene expression data from thalidomide modulated- and unmodulated-MSCs from patients with idiopathic thrombocytopenic purpura (ITP). This experiment consists of 3 arrays.
Project description:Whole blood transcriptional profiling of pediatric idiopathic thrombocytopenic purpura (ITP) patients comparing self-limited acute ITP patients with chronic ITP patients or progression-to-chronic ITP patients. Patient samples were collected during 3 different disease states: 8 samples from acute ITP pts (within 6 months of dx, during active disease), 4 samples from progression-to-chronic ITP pts (within 6 months of dx), and 14 samples from chronic ITP (after 6 months of dx).
Project description:Idiopathic thrombocytopenic purpura (lTP) is anautoimmune disorder characterized by antiplateletautoantibodies, thrombocytopenia, and bleeding. In thisstudy, a kind of active component, chlorogenic acidcompounds(CGAs)from sweetpotato leaves was isolatedby ionic liquids-ultrasound, which contains 6 isomers ofchlorogenic acid with total purity of 95.69%. CGAs couldpromote platelet formation in a murine model of ITP, whichwas established by injection of antiplatelet antibody. Tocharacterize the impact of CGAs on the bone marrowproteome in mice, tissue samples were subjected toproteomic analysis.
Project description:Idiopathic thrombocytopenic purpura (ITP) is an autoimmune disorder characterized by antiplatelet autoantibodies, thrombocytopenia, and bleeding, however, its treatment options are limited. In this study, a kind of active component, caffeoylquinic acid compounds from sweetpotato leaves was extracted out to explore its medicinal value and provide novel therapeutic strategies for the treatment of ITP. We isolated three compounds from the n-butanol extract of sweetpotato leaves. Caffeoylquinic acids could participate the immune balance of T-helper 17 lymphocytes (Th17) / regulatory T cells (Treg) in a murine model of ITP, which was established by injection of antiplatelet antibody. Further quantitative proteomics analysis revealed that caffeoylquinic acid could activate AMPK signaling pathway. It suggested that caffeoylquinic acid may provide a novel therapeutic strategy that relies on the AMPK pathway.
