Dataset Information

Activation of immediate early response genes initiates a restorative gene expression program during each arousal from torpor in hibernating 13-lined ground squirrels

ABSTRACT: Activation of immediate early response genes initiates a restorative gene expression program during each arousal from torpor in hibernating 13-lined ground squirrels

PROVIDER: PRJNA854159 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	SRR19901702.fastq.gz	Fastqsanger.gz
	SRR19901703.fastq.gz	Fastqsanger.gz
	SRR19901704.fastq.gz	Fastqsanger.gz
	SRR19901705.fastq.gz	Fastqsanger.gz
	SRR19901706.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 31

Similar Datasets

Project description:Hibernation is energy saving adaptation involving suppression of activity to survive in highly seasonal environments. Immobility and disuse generate muscle loss in most mammalian species. In contrast to other mammals, bears and ground squirrels demonstrate limited muscle atrophy over the physical inactivity of winter hibernation. This suggests that hibernating mammals have adaptive mechanisms to prevent disuse muscle atrophy. To identify common transcriptional program underlying molecular mechanisms preventing muscle loss, we conducted a large-scale gene expression screening in hind limb muscles comparing hibernating and summer active black bears and arctic ground squirrels by the use of custom 9,600 probe cDNA microarrays. The molecular pathway analysis showed an elevated proportion of overexpressed genes involved in all stages of protein biosynthesis and ribosome biogenesis in muscle of both species during hibernation that implies induction of translation at different hibernation states. The induction of protein biosynthesis likely contributes to attenuation of disuse muscle atrophy through prolonged periods of immobility and starvation. This adaptive mechanism allows hibernating mammals to maintain full musculoskeletal function and preserve mobility during and immediately after hibernation, thus promoting survival. The lack of directional changes in genes of protein catabolic pathways does not support the importance of metabolic suppression for preserving muscle mass during winter. Coordinated reduction of multiply genes involved in oxidation reduction and glucose metabolism detected in both species is consistent with metabolic suppression and lower energy demand in skeletal muscle during inactivity of hibernation. Arctic ground squirrels sampled during winter hibernation were compared with the animals sampled during summer. Muscle was hybridized on a custom 9,600 probes nylon membrane microarray platform. Ten in late torpor, four in early arousal, then in late arousal were studied in experiments.

Dataset Information

Activation of immediate early response genes initiates a restorative gene expression program during each arousal from torpor in hibernating 13-lined ground squirrels

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets