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M1BP RNAi during Drosophila flight myogenesis: Analyses at 0h APF stage

ABSTRACT: M1BP RNAi during Drosophila flight myogenesis: Analyses at 0h APF stage

PROVIDER: PRJNA854350 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR19906223.fastq.gz	Fastqsanger.gz
	SRR19906224.fastq.gz	Fastqsanger.gz
	SRR19906225.fastq.gz	Fastqsanger.gz
	SRR19906226.fastq.gz	Fastqsanger.gz
	SRR19906227.fastq.gz	Fastqsanger.gz

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M1BP RNAi during Drosophila flight myogenesis: Analyses at 0h APF stage

Project description:Oxidative metabolism is the predominant energy source for muscle contraction. How this is transcriptionally regulated during muscle development to accommodate different metabolic requirements and muscle types is largely unknown. We show that the formation of mitochondria cristae harbouring the respiratory chain is concomitant with a large-scale transcriptional upregulation of genes linked with oxidative phosphorylation (OXPHOS) during the middle of Drosophila flight muscle development. We further demonstrate using high-resolution imaging, transcriptomic and biochemical analyses that Motif-1-binding protein (M1BP) transcriptionally regulates the expression of genes encoding critical components for OXPHOS complex assembly and integrity. In the absence of M1BP, mitochondrial respiratory complexes are improperly assembled and aggregate in the mitochondrial matrix, triggering a strong protein quality control response. Together, this study provides the first insight into the transcriptional regulation of oxidative metabolism during Drosophila myogenesis and identifies M1BP as a novel player in this process.

2023-05-12 | GSE207239 | GEO

M1BP RNAi during Drosophila flight myogenesis: Analyses at 48h APF stage

Project description:M1BP RNAi during Drosophila flight myogenesis: Analyses at 48h APF stage

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M1BP RNAi during Drosophila flight myogenesis: Analyses at 64h APF stage

Project description:M1BP RNAi during Drosophila flight myogenesis: Analyses at 64h APF stage

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M1BP RNAi during Drosophila flight myogenesis: Analyses at 64h APF stage

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M1BP RNAi during Drosophila flight myogenesis: Analyses at 48h APF stage

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M1BP RNAi during Drosophila flight myogenesis: Analyses at 2-day adult stage

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M1BP RNAi during Drosophila flight myogenesis: Analyses at 2-day adult stage

Project description:M1BP RNAi during Drosophila flight myogenesis: Analyses at 2-day adult stage

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The RNA binding protein Arrest (Aret) regulates myofibril maturation in Drosophila flight muscle

Project description:In Drosophila, fibrillar flight muscles (IFMs) enable flight, while tubular muscles mediate other body movements. Here, we use RNA-sequencing and isoform-specific reporters to show that spalt major (salm) determines fibrillar muscle physiology by regulating transcription and alternative splicing of a large set of sarcomeric proteins. We identify the RNA binding protein Arrest (Aret, Bruno) as downstream of salm. Aret shuttles between cytoplasm and nuclei, and is essential for myofibril maturation and sarcomere growth of IFMs. Molecularly, Aret regulates IFM-specific transcription and splicing of various sarcomeric targets, including Stretchin and wupA (TnI), and thus maintains muscle fiber integrity. As Aret and its sarcomeric targets are evolutionarily conserved, similar principles may regulate mammalian muscle morphogenesis. 9 samples from Drosophila melanogaster were analyzed in duplicate: control dissected wildtype flight muscle at 30h APF, 72h APF and 0 day adult, jump muscle and whole leg from 1d adult and RNAi/mutant conditions for salm (1d flight muscle) and aret (30h, 72h and 1d flight muscle)

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ChIP-seq analysis of M1BP in Drosophila S2R+ cells

Project description:The genomic distribution of a novel transcription factor called M1BP was determined in Drosophila S2R+ cells Polyclonal antibody raised against M1BP was used to immunoprecipitate M1BP-DNA adducts generated by treating Drosophila cells with formaldehyde, lysing the cells, and shearing DNA by sonication. Immunoprecipitated DNA was sequenced using the AB SOLiD system.

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Expression analysis of Drosophila S2R+ cells depleted of M1BP by RNAi

Project description:Microarray analysis of gene expression profiles in control and M1BP-depleted Drosophila S2R+ cells. The results of this analyses are reported in Li, J. and D.S. Gilmour (2013) The newly identified transcription factor M1BP ad GAGA factor orchestrate distinct mechanisms of transcriptional regulation on paused genes. The EMBO J, in press. Four biological replicates comparing total RNA isolated from M1BP-RNAi treated and LacZ-RNAi treated cells.

2013-08-13 | E-GEOD-46629 | biostudies-arrayexpress

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