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Transcriptomic profile at single cell level of Age-associated B cells (ABCs) from individuals with different health conditions

ABSTRACT: Transcriptomic profile at single cell level of Age-associated B cells (ABCs) from individuals with different health conditions

PROVIDER: PRJNA855895 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR20020800.fastq.gz	Fastqsanger.gz
	SRR20020801.fastq.gz	Fastqsanger.gz
	SRR20020802.fastq.gz	Fastqsanger.gz
	SRR20020803.fastq.gz	Fastqsanger.gz
	SRR20020804.fastq.gz	Fastqsanger.gz

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Transcriptomic profile at single cell level of Age-associated B cells (ABCs) from individuals with different health conditions

Project description:Age-associated B cells are considered a homogenoeus B cell sub-population whose accumulation has been linked to a altered immune response. We used single cell RNA sequencing (scRNA-seq) to analyze the diversity ABCs from different health conditions.

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Sequencing of cfDNA derived from the plasma of individuals of different ages

Project description:We provide the first in vivo evidence of global and local chromatin changes in human aging by analyzing cfDNA from the blood of individuals of different age groups. Our results show that nucleosome signals inferred from cfDNA are consistent with the redistribution of heterochromatin observed in cellular senescence and aging in other model systems. It also revealed age and deteriorating health status correlate with a low-level enrichment of signals from cells in the thyroid gland. In addition, we detected an overall nucleosome loss at several genomic locations, such as transcription start and termination sites, 5’UTR of L1HS retrotransposons and dimeric AluY elements with age.

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