Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Elements of divergence in germline determination in closely related sea urchin species

ABSTRACT: Elements of divergence in germline determination in closely related sea urchin species

PROVIDER: PRJNA860641 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR20336815_1.fastq.gz	Fastqsanger.gz
	SRR20336815_2.fastq.gz	Fastqsanger.gz
	SRR20336816_1.fastq.gz	Fastqsanger.gz
	SRR20336816_2.fastq.gz	Fastqsanger.gz
	SRR20336817_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 24

Similar Datasets

Elements of divergence in germline determination in closely related sea urchin species

Project description:Evolutionary transitions enable the wide diversity in life histories of plants and animals. This is particularly germane in the development of the germline in which fitness is a direct readout of evolutionary change. Here, we focused on two distinct sea urchin species who shared a common ancestor 50 million years ago. Even though they both rely on inherited mechanisms to specify their germ line, the integration of stage-matched scRNA seq datasets from these two sea urchins revealed, amongst others, a broader expression of Nanos2 in Lytechinus variegatus (Lv) compared to Strongylocentrotus purpuratus (Sp). In Sp, Nanos2 mRNA expression is highly restricted to the PGCs by a lability element in its 3’UTR. This element is lacking in Lv Nanos2, explaining how this mRNA more broadly accumulates in the Lv embryos. We discovered that the Lv Nanos2 3’UTR instead leads to a germline specific translation of the protein. The results emphasize that regulatory mechanisms resulting in germline diversity rely less on transcriptional regulation and more on post-transcriptional and post-translational restrictions of key gene products, such as Nanos2.

2023-01-19 | GSE208709 | GEO

sea urchin metagenome

Project description:Sea urchin microbiome

| PRJDB12341 | ENA

Elements of divergence in germline determination in closely related species.

Project description:Evolutionary transitions are particularly important in development of the germ line, cells which directly impact sexual reproduction. Differences in the primordial germ cells (PGCs) of two sea urchin species were examined here by stage-matched, integrated, single cell RNA-seq (scRNA-seq) datasets. Even though both species rely on inherited mechanisms to specify their germ line, this analysis revealed a variety of differences in germline gene expression, including a broader expression of the germline factor Nanos2 (Nan2) in Lytechinus variegatus (Lv) compared to Strongylocentrotus purpuratus (Sp). In Sp, Nan2 mRNA expression is highly restricted to the PGCs by a lability element in its 3'UTR, which is lacking in the mRNA of Lv-Nan2, thus explaining the difference. We discovered that the Lv-Nan2 3'UTR instead leads to its specific translation in the PGCs. The results emphasize that regulatory mechanisms resulting in germline specification rely greatly on post-transcriptional restrictions of key gene products.

| S-EPMC10068562 | biostudies-literature

Base-resolution DNA methylation maps of purple sea urchin (Strongylocentrotus purpuratus)

Project description:Using MethylC-seq we investigated single-base resolution methylomes of sea urchin during development.

2021-11-08 | GSE188333 | GEO

Gene expression alterations in SoxC knockdown sea urchin embryos

2015-12-16 | GSE76067 | GEO

Gene expression alterations in SoxC knockdown sea urchin embryos

Project description:Transcription factor SoxC is required for all neural development in purple sea urchin S. purpuratus embryos. To begin to develop a gene regulatory network for neural development, we used RNA-Seq to compare transcript populations in SoxC knockdown and control embryos. SoxC function was knocked down by morpholino oligo injection. RNA from about 1000 embryos were collected for both control and knockdown samples.

2015-12-16 | E-GEOD-76067 | biostudies-arrayexpress

Developmental dynamics of sea urchin and sea star cis-regulation and the evolution of echinoderm genome organization

Project description:We identified cis-regulatory elements based on their dynamic chromatin accessibility during the gastrula-larva stages of sea urchin and sea star and studied their evolution in these echinoderm species

2021-10-24 | GSE186363 | GEO

The sea urchin larval response to immune challenge with Vibrio diazotrophicus

Project description:Seawater exposure to the gram negative marine bacterium Vibrio diazotrophicus induces a robust cellular response in sea urchin larvae that includes the migration of pigment cells to the gut epithelium, changes in cell behavior and altered gut morphology (Ho et al., 2016; PMID 27192936). To investigate the transcriptional underpinnings of this response, whole transcriptome sequencing was performed on mRNA isolated from larval samples collected at 0, 6, 12 and 24 hr of exposure to V. diazotrophicus. The morphological simplicity of the sea urchin larva provides a systems-level model for identifying biologically relevant transcriptional state changes in response to dysbiosis in the gut lumen.

2017-03-24 | GSE96940 | GEO

sea urchin metagenome

Project description:Bacterial microbiota associated with the coelomic fluid of the sea urchin Paracentrotus lividus

| PRJNA328923 | ENA

Proteomic analysis of sea urchin egg provisioning and development

Project description:Here we present LC-MS/MS proteomic datasets of Heliocidaris erythrogramma, Heliocidaris tuberculata, and Lytechinus variegatus eggs and larvae. We find dramatic proteomic differences likely associated with life history evolution and between developmental stages. This study provides a complimentary dataset to previous transcriptomic analyses of the same three sea urchin species.

2019-03-04 | PXD007065 | Pride