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ABSTRACT: Apis mellifera Linnaeus head

PROVIDER: PRJNA866287 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR20852100_1.fastq.gz	Fastqsanger.gz
	SRR20852100_2.fastq.gz	Fastqsanger.gz
	SRR20852101_1.fastq.gz	Fastqsanger.gz
	SRR20852101_2.fastq.gz	Fastqsanger.gz
	SRR20852102_1.fastq.gz	Fastqsanger.gz

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Transcription profiling by array of brains from Apis mellifera mellifera and Apis mellifera ligustica honey bees of different ages

Project description:Expression profiling of honey bees brains as a function of genotype ( Apis mellifera mellifera/Apis mellifera ligustica) and age

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MicroRNA expression profile of Apis mellifera ligustica brain tissue

Project description:We analyzed the changes in the brain tissue of Apis mellifera ligustica at the molecular level by sequencing after using fluvalinate. We found that the differentially expressed miRNAs (DEM) may be involved in hippocampal cell apoptosis and damage to memory functions. This result may be related to behaviors observed after the administration of this medication, such as a lack of homing at night and behavioral disturbances. Overall, our results provide new information about the molecular mechanisms and pathways of fluvalinate action in the brain tissue of Apis mellifera ligustica.

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Project description:Surface Microorganisms of Apis cerana cerana and Apis mellifera Linnaeus

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Effects of fat body microRNA miR-305-5p knockdown on brain and fat body gene expression in Apis mellifera

Project description:RNA sequencing of Apis mellifera abdominal fat body and matched whole brain following a knockdown in fat body ame-miR-305-5p expression

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Comparative methylomics reveals gene-body H3K36me3 in Drosophila predicts DNA methylation and CpG landscapes in other invertebrates

Project description:Here, we demonstrate that Nematostella vectensis, Ciona intestinalis, Apis mellifera, and B. mori, show two distinct populations of genes differentiated by gene-body CpG density. Genome-scale DNA methylation profiles for A. mellifera spermatozoa reveal CpG-poor genes are methylated in the germ line, as predicted by the depletion of CpGs. We find an evolutionarily conserved distinction between CpG-poor and -rich genes: the former are associated with basic biological processes, the latter with more specialized functions. This distinction is strikingly similar to that recently observed between euchromatin-associated genes in Drosophila that contain intragenic histone 3 lysine 36 trimethylation (H3K36me3) and those that do not, even though Drosophila doesnM-CM-"M-BM-^@M-BM-^Yt display CpG density bimodality or methylation. We confirm that a significant number of CpG-poor genes in N. vectensis, C. intestinalis, A. mellifera and B. mori are orthologs of H3K36me3- rich genes in Drosophila. We propose that over evolutionary time, gene-body H3K36me3 has influenced gene-body DNA methylation levels, and consequently the gene-body CpG density bimodality characteristic of invertebrates that harbor CpG methylation. Examination of DNA methylation in Apis Mellifera sperm

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Transcriptional changes in brain and abdominal tissue in response to Vitellogenin knockdown

Project description:Investigation into changes in gene expression in Apis mellifera using RNAi to knockdown Vg expression

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Proteomic comparison of summer and winter Apis mellifera workers shows reduced protein abundance in winter samples

Project description:Apis mellifera workers in temperate climates display two castes; short lived summer bees that engage in nursing, hive maintenance and foraging, and long lived winter bees (diutinus bees) which remain within the hive and are essential for thermoregulation. Label free quantitative proteomic analysis was conducted on A. mellifera workers sampled in June and December to compare the proteomes of summer and winter bees. Proteomic analysis was completed on head, abdominal and venom sac samples which revealed an elevated level of protein abundance in summer bees but and a decrease in protein abundance in winter bees. Head and abdominal samples displayed an increase in cuticular proteins in summer samples whereas an increase in xenobiotic proteins was observed in winter samples. Several carbohydrate metabolism pathways which have been linked to energy production and longevity in insects were observed to be increased in abundance in winter samples in comparison to summer samples. Proteomic analysis of the venom sacs an increased abundance and expression of bee venom associated proteins in comparison to winter workers. These data provides an insight into the adaptions of A. mellifera workers in summer and winter and may aid in future treatment and disease studies on honeybee colonies.

2022-05-20 | PXD030483 | Pride

Proteomic comparison of summer and winter Apis mellifera workers shows reduced protein abundance in winter samples

2022-11-21 | PXD030481 | Pride

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