Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression of human bone marrow-derived stem cells on liquid crystalline self-assembed hydroxyapatite nanorods for bone regeneration

ABSTRACT: Gene expression of human bone marrow-derived stem cells on liquid crystalline self-assembed hydroxyapatite nanorods for bone regeneration

PROVIDER: PRJNA867161 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR20958311_1.fastq.gz	Fastqsanger.gz
	SRR20958311_2.fastq.gz	Fastqsanger.gz
	SRR20958312_1.fastq.gz	Fastqsanger.gz
	SRR20958312_2.fastq.gz	Fastqsanger.gz
	SRR20958313_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 30

Similar Datasets

Gene expression of human bone marrow-derived stem cells on liquid crystalline self-assembed hydroxyapatite nanorods for bone regeneration

Project description:Liquid crystal-like, low-dimensional and long-range ordered structures (LC) are found in human tissues including bones. The excellent mechanical and biological functions of natural bones entail the well-orchestrated nano-scale hydroxyapatite (HAp) crystals within collagen fibres matrix. However, the understanding of the self-assembling of HAp nanocrystals and formation of hierarchically ordered bone structure are still elusive. Our study aims to develop an in situ traceable anisotropic HAp nanorods doped with citrate and terbium (Cit/Tb-HAp NRs), investigate their self-assembling behaviour and illuminate their potential and pathway of guiding ordered osteogenesis at multi-scale in vitro.

2024-08-23 | GSE210696 | GEO

Comparative transcriptomic analysis of HUVECs cultured on hydroxyapatite doping with Mg2+ versus Trio-Active bone scaffold

Project description:Comparative transcriptomic analysis of HUVECs cultured on hydroxyapatite doping with Mg2+ (HA/Mg) versus Trio-Active bone scaffold (TABS)

2026-01-06 | GSE315261 | GEO

Comparative transcriptomic analysis of Raw264.7 cells cultured on hydroxyapatite doping with Mg2+ versus Trio-Active bone scaffold

Project description:Comparative transcriptomic analysis of Raw264.7 cells cultured on hydroxyapatite doping with Mg2+ (HA/Mg) versus Trio-Active bone scaffold (TABS)

2026-01-06 | GSE315260 | GEO

Liquid Crystalline Hydroxyapatite Nanorods Orchestrate Hierarchical Bone-Like Mineralization.

Project description:Bone matrix exhibits exceptional mechanical properties due to its unique nanocomposite structure of type I collagen fibrils and hydroxyapatite (HAp) nanoparticles in hierarchical liquid crystalline (LC) order. However, the regeneration mechanism of this LC structure is elusive. This study investigates the role of the LC structure of HAp nanorods in guiding aligned mineralization and its underlying molecular mechanism. A unidirectionally oriented LC phase of HAp nanorods is developed through engineering-assisted self-assembling. This is used to study the growth direction of long-range aligned extracellular matrix (ECM) and calcium deposit formation during the osteogenic differentiation of human bone marrow-derived mesenchymal stem cells. It is found that 2 key regulatory genes, COL1A1 and COL4A6, lead to the formation of aligned ECM. Activation of the PI3K-Akt pathway enhances osteogenesis and promotes ordered calcium deposits. This study provides evidence for elucidating the mechanism of LC-induced ordered calcium deposition at hierarchical levels spanning from the molecular to macro-scale, as well as the switch from ordered to disordered mineralization. These findings illuminate bone regeneration, contribute to the development of biomimetic artificial bone with long-range ordered structures, and suggest a basis for therapeutic targeting of microstructure-affected bone disorders and the broader field of cell-ECM interactions.

| S-EPMC11673523 | biostudies-literature

Comparative transcriptomic analysis of MC3T3-E1 cells cultured on hydroxyapatite doping with Mg2+ versus Trio-Active bone scaffold

Project description:Comparative transcriptomic analysis of MC3T3-E1 cells cultured on hydroxyapatite doping with Mg2+ (HA/Mg) versus Trio-Active bone scaffold (TABS)

2026-01-06 | GSE315259 | GEO

Transcriptomic profiling of osteoblasts on hydroxyapatite-coated surfaces reveals enhanced osteogenic and angiogenic processes relevant to accelerated bone healing.

Project description:Hydroxyapatite (HA) coating on orthopedic implants is known to enhance osteogenesis and bone-implant integration. However its molecular basis remains to be investiagated. In this study, we performed transcriptome analysis of osteoblasts on nano-HA-coated surfaces.

2026-03-16 | GSE309449 | GEO

3D-Printed GelMA/Hydroxyapatite/Barium Titanate Piezoelectric Hydrogels for Bone Tissue Engineering

Project description:Bone defect repair remains a major clinical challenge. This study presents a novel strategy using a 3D-printed piezoelectric hydrogel scaffold—composed of gelatin methacrylate (GelMA), hydroxyapatite (HA), and barium titanate (BTO)—for functional bone tissue engineering. The GelMA/HA/BTO (GelHABT) scaffold exhibited a well-defined porous structure, enhanced mechanical stability, and, crucially, reliable piezoelectric responsiveness. This key feature enables the material to convert external mechanical stimuli, such as low-intensity pulsed ultrasound (LIPUS), into endogenous electrical signals. In vitro, the scaffold promoted BMSCs adhesion, proliferation, and osteogenic differentiation, with performance significantly enhanced under LIPUS stimulation. Mechanistic insights revealed that the piezoelectric microenvironment remodeled the cellular miRNA expression profile, particularly up-regulating osteogenesis-related miR-29b-3p and activating the AMPK signaling pathway. Collectively, this ultrasound-responsive, gene-regulating scaffold represents a promising approach for treating bone defects by leveraging piezoelectricity to actively stimulate bone regeneration.

2026-01-28 | GSE317440 | GEO

3D-Printed GelMA/Hydroxyapatite/Barium Titanate Piezoelectric Hydrogels for Bone Tissue Engineering

Project description:3D-Printed GelMA/Hydroxyapatite/Barium Titanate Piezoelectric Hydrogels for Bone Tissue Engineering

| PRJNA1412785 | ENA

Effect of nano-hydroxyapatite and phosphate on thorium toxicity – Arabidopsis transcriptomic study

Project description:Thorium (Th) is a ubiquitous element but elevated concentrations caused by anthropogenic or natural activities pose health and environmental risks. Nowadays, Th is obtained mainly as by-product during the mining and extraction rare earth element. However, due to its abundance in nature, which is higher than the abundance of uranium, Th is considered as a potential fuel for the reactors. Therefore, in this study, we focused on the effect of thorium on Arabidopsis plants at the transcriptomic level. Since Th and phosphorus form insoluble phosphates, we focused on the effect of Th in the presence/absence of phosphate. Furthermore, we investigated the effect of hydroxyapatite nanoparticles, which are considered as material for in situ remediation, on Th toxicity. Not surprisingly, the highest number of DEGs was recorded in the combination of P absence and Th presence followed by the presence of Th in the standard hydroponic medium, the absence of P in the medium, the presence of nano-hydroxyapatite, and the presence of Th together with hydroxyapatite. Besides a high number of genes involved in stress response, Th also induced the transcription of genes involved in response to P starvation, indicating that Th decreased the availability of P. P deficiency caused by Th contributed to the phytotoxic effect of Th. Nano-hydroxyapatite significantly reduced Th toxicity. Nano-hydroxyapatite also served as a source of inorganic P for the plants, but caused deficiency of essential metals indicated by up-regulation of genes involved in their acquisition.

2023-06-24 | GSE233480 | GEO

Mechanisms of crystalline silica-induced pulmonary toxicity revealed by global gene expression profiling

Project description:This SuperSeries is composed of the following subset Series: GSE30178: Mechanisms of crystalline silica-induced pulmonary toxicity revealed by global gene expression profiling (rat lungs) GSE30180: Mechanisms of crystalline silica-induced pulmonary toxicity revealed by global gene expression profiling (A549 cells dataset 1) GSE30200: Mechanisms of crystalline silica-induced pulmonary toxicity revealed by global gene expression profiling (A549 cells dataset 2) GSE30213: Mechanisms of crystalline silica-induced pulmonary toxicity revealed by global gene expression profiling (A549 cells dataset 3) GSE30214: Mechanisms of crystalline silica-induced pulmonary toxicity revealed by global gene expression profiling (A549 cells dataset 4) GSE30215: Mechanisms of crystalline silica-induced pulmonary toxicity revealed by global gene expression profiling (A549 cells dataset 5) Refer to individual Series

2012-06-29 | E-GEOD-30216 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data