Project description:Eggs and juveniles of NWE-Mc31 from Meloidogyne chitwoodi were collected and total RNA was isolated as described in the sample collection protocol. The quality of both RNA isolations was RIN 10 (RNA Integrity Number). mRNA sequences were generated using the TruSeq RNA stranded polyA protocol of Useq (Utrecht Sequencing Facility, Utrecht, The Netherlands.
Project description:In this experiment we sequence the whole genome DNA of 28 unique populations of the root-knot nematode Meloidogyne hapla to investigate their genetic diversity using illumina sequencing. The hapla populations came from various locations, mostly in the Netherlands and Europe. The nematodes were maintained on tomato cv. Money Maker in the greenhouse of Wageningen University. After DNA extraction, DNA was sent for sequencing. The resulting sequence was used for calling variants and genetic analysis.
