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Epigenetic reprogramming of intestinal stemness by dietary arachidonic acid [In_vivo]

ABSTRACT: Epigenetic reprogramming of intestinal stemness by dietary arachidonic acid [In_vivo]

PROVIDER: PRJNA867325 | ENA |

REPOSITORIES: ENA

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Epigenetic reprogramming of intestinal stemness by dietary arachidonic acid

Project description:Epigenetic reprogramming of intestinal stemness by dietary arachidonic acid

| PRJNA867320 | ENA

Epigenetic reprogramming of intestinal stemness by dietary arachidonic acid [Organoid]

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Epigenetic reprogramming of intestinal stemness by dietary arachidonic acid [intestinal organoids]

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Epigenetic reprogramming of intestinal stemness by dietary arachidonic acid [CutnRun 1]

Project description:We evaluated tthe necessity of PGE2-Ptger4 signaling in regulating stemness enhancing effects of arachidonic acid (AA) via CutnRun and found that Ptger4 KO organoids failed to cause global methylation changes in signature genes that are associated with stem cell reprogramming in response to AA or PGE2.

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Epigenetic reprogramming of intestinal stemness by dietary arachidonic acid [CutnRun 1]

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Epigenetic reprogramming of intestinal stemness by dietary arachidonic acid [ATAC-Seq]

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Epigenetic reprogramming of intestinal stemness by dietary arachidonic acid [CutnRun 2]

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Epigenetic reprogramming of intestinal stemness by dietary arachidonic acid [intestinal organoids]

Project description:We evaluated the effects of arachidonic acid (AA) on intestinal stem cell regeneration by RNA-seq and found that AA treatment led to strong upregulation of genes that are associated with stem cell reprogramming across the time points. AA elicits a conserved stem cell regeneration program in mouse intestinal organoids.

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Epigenetic reprogramming of intestinal stemness by dietary arachidonic acid [ATAC-Seq]

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