Project description:Characterization of miRNAs in red flour beetle Tribolium castaneum by deep sequencing of two different RNA libraries. Sequencing of Tribolium small RNAs from adults and embryos.
Project description:Genome-wide survey of transcriptional differences between males and females of Tribolium castaneum, the red flour beetle Four biological replicates for male and female beetles with 20 individuals per replicate. Two technical replicates, one replicate per sex. 16,434 genes/expressed non-coding regions represented twice on each array. Three 60 mer probes for most exons/expressed non-coding regions. 167,538 unique genomic probes replicated twice per array.
Project description:This work defines the conserved core of TFs involved in insect brain NB specification by cross-species comparison of single-cell transcriptomics of NBs from, the red flour beetle, Tribolium castaneum, and the vinegar fly, Drosophila melanogaster. In Tribolium, we established a Gal4 enhancer trap screen system to generate a line that marks NBs. Using the 10x genomics technology, we sequenced 37,137 Tribolium NBs. We also sequenced 32,112 single nuclei of Drosophila NBs. Cross-species comparison followed by in situ hybridization led to the identification brain-specific TFs of insects. This brain NB-specific gene set confirms fundamentally different spatial patterning compared to the well-studied ventral nerve cord.
Project description:This series examines gene expression in the anterior midgut at several time points (2, 4, 8, & 16 h) after topical application of juvenile hormone III (JHIII) or acetone (control) to adult beetles of both sexes. Keywords = North American pine engraver beetle Keywords = anterior midgut Keywords = juvenile hormone Keywords = pheromone biosynthesis Keywords = Coleoptera Keywords = Scolytidae Keywords: other