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RNA transcriptome sequencing analysis of SNU-668 Erastin-resistant cells transfected with shRNA-SOX13 or control shRNA

ABSTRACT: RNA transcriptome sequencing analysis of SNU-668 Erastin-resistant cells transfected with shRNA-SOX13 or control shRNA

PROVIDER: PRJNA868768 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR21019259_1.fastq.gz	Fastqsanger.gz
	SRR21019259_2.fastq.gz	Fastqsanger.gz
	SRR21019260_1.fastq.gz	Fastqsanger.gz
	SRR21019260_2.fastq.gz	Fastqsanger.gz
	SRR21019261_1.fastq.gz	Fastqsanger.gz

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RNA transcriptome sequencing analysis of SNU-668 Erastin-resistant cells transfected with shRNA-SOX13 or control shRNA

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Ribo-seq analysis analysis of SNU-668 Erastin-resistant cells transfected with shRNA-ALKBH3 or control shRNA

Project description:This study presents a comprehensive ribosome profiling (Ribo-seq) analysis conducted on SNU-668 gastric cancer cells with acquired resistance to Erastin. These cells were genetically modified through stable transfection with either a short hairpin RNA (shRNA) construct specifically targeting and knocking down the expression of the ALKBH3 gene, or a corresponding non-targeting control shRNA, to systematically investigate the consequent alterations in global translational activity.

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m1A enrichment analysis of SNU-668 Erastin-resistant cells transfected with shRNA-ALKBH3 or control shRNA

Project description:An m1A enrichment analysis was conducted to investigate the epigenetic landscape and RNA modification profiles within SNU-668 Erastin-resistant gastric cancer cells following the experimental manipulation of ALKBH3 expression. Specifically, this analysis compared cells that had been stably transfected with a targeted shRNA construct designed to knock down ALKBH3 against control cells transfected with a non-targeting scramble shRNA sequence. The overarching goal was to assess and quantify the differential enrichment patterns of N1-methyladenosine (m1A) modifications across the transcriptome, thereby elucidating the potential regulatory role of the ALKBH3 demethylase in modulating the epitranscriptomic state underlying the acquired Erastin-resistant phenotype in this cellular model.

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RNA transcriptome sequencing analysis of SNU-668 Erastin-resistant cells transfected with shRNA-SPIN1 or control shRNA

Project description:An RNA transcriptome sequencing analysis was performed in SNU-668 erastin-resistant cells to investigate their molecular profile. To assess the specific role of the SPIN1 protein, a comparative approach was undertaken. This involved transfecting the cells with either a targeted shRNA construct against SPIN1 or, for baseline comparison, a non-targeting control shRNA. The subsequent sequencing data were then generated to enable a detailed comparison of gene expression patterns between these two experimental conditions, aiming to identify key transcriptional changes associated with SPIN1 knockdown in the context of ferroptosis resistance.

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