Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Genomic occupation profile of Mediator MED23 in 293T cells

ABSTRACT: Genomic occupation profile of Mediator MED23 in 293T cells

PROVIDER: PRJNA868785 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR21019716_1.fastq.gz	Fastqsanger.gz
	SRR21019716_2.fastq.gz	Fastqsanger.gz
	SRR21019717_1.fastq.gz	Fastqsanger.gz
	SRR21019717_2.fastq.gz	Fastqsanger.gz
	SRR21019718_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 8

Similar Datasets

Genomic occupation profile of Mediator MED23 in 293T cells

Project description:We performed CUT&Tag experiments in MED23-HA and WT 293T cells to analyze the genomic occupation profiles for the Mediator MED23.

2025-05-28 | GSE211082 | GEO

The occupied profile of FIP1 in WT and MED23-KD 293T cells

Project description:MED23-KD 293T cells in which MED23 was mostly knockouted were generated by CRISPR-Cas9. We performed ChIP experiments in WT and MED23-KD cells to profile the genomic occupation profiles of FIP1, which is one of the components of pre-mRNA 3' end processing complex.

2025-05-28 | GSE211080 | GEO

RNA binding profile of MED23 in 293T cells

Project description:To delineate the repertoire of RNA binding of MED23, we performed a denatured CLIP-seq experiment coupled with high-salt wash in MED23-HA 293T cells and wildtype 293T cells as a control.

2025-05-28 | GSE211081 | GEO

RNA polymerase II binding profile in WT and MED23-KD 293T cells

Project description:MED23-KD 293T cells in which MED23 was mostly knockouted were generated by CRISPR-Cas9. We performed CUT&Tag experiments in WT and MED23-KD cells to characterize the change of Pol II binding.

2025-05-28 | GSE211084 | GEO

Transcription profiles of MED23-KD and wildtype 293T cells

Project description:To characterize the function of MED23 on transcription, we generated two MED23-KD 293T cell lines in which MED23 was about 75% knockouted by CRISPR-Cas9. Then we performed RNA-seq to analyze the change of transcript levels around termination sites.

2025-05-28 | GSE211083 | GEO

Mediator Complex Regulates Alternative mRNA Processing via the Med23 Subunit

Project description:Mediator complex is an integrative hub for transcriptional regulation. Here we show that Mediator regulates alternative mRNA processing via its Med23 subunit. Combining tandem affinity purification and mass spectrometry, we identified a number of mRNA processing factors that bind to a soluble recombinant Mediator subunit MED23 but not to several other Mediator components. One of these factors, hnRNP L, specifically interacts with MED23 in vitro and in vivo. Consistently, Mediator partially colocalizes with hnRNP L and the splicing machinery in the cell. Functionally Med23 regulates a subset of hnRNP L-targeted alternative splicing (AS) and alternative cleavage and polyadenylation (APA) events as shown by minigene reporters and exon array analysis. ChIP-seq analysis revealed that Med23 can regulate hnRNP L occupancy at their co-regulated genes. Taken together, these results demonstrate a crosstalk between Mediator and the splicing machinery, suggesting a novel mechanism for coupling mRNA processing to transcription. Examination of hnRNP L and H3K36me3 enrichment in sictrl and si23 Hela cells

2012-02-24 | E-GEOD-33887 | biostudies-arrayexpress

Mediator Complex Regulates Alternative mRNA Processing via the Med23 Subunit

Project description:Mediator complex is an integrative hub for transcriptional regulation. Here we show that Mediator regulates alternative mRNA processing via its Med23 subunit. Combining tandem affinity purification and mass spectrometry, we identified a number of mRNA processing factors that bind to a soluble recombinant Mediator subunit MED23 but not to several other Mediator components. One of these factors, hnRNP L, specifically interacts with MED23 in vitro and in vivo. Consistently, Mediator partially colocalizes with hnRNP L and the splicing machinery in the cell. Functionally Med23 regulates a subset of hnRNP L-targeted alternative splicing (AS) and alternative cleavage and polyadenylation (APA) events as shown by minigene reporters and exon array analysis. ChIP-seq analysis revealed that Med23 can regulate hnRNP L occupancy at their co-regulated genes. Taken together, these results demonstrate a crosstalk between Mediator and the splicing machinery, suggesting a novel mechanism for coupling mRNA processing to transcription. We performed an exon array experiment using HeLa cells expressing Med23, hnRNP L or control siRNAs which were established by a virus-mediated siRNA technology. Each sample was done in three biological replicates. Total RNA of these cell lines was processed and hybridized to the Affymetrix human exon array.

2012-02-24 | E-GEOD-33771 | biostudies-arrayexpress

RNA binding profile of MED23 in 293T cells

Project description:RNA binding profile of MED23 in 293T cells

| PRJNA868786 | ENA

Mediator Complex Regulates Alternative mRNA Processing via the Med23 Subunit

2012-02-24 | GSE33887 | GEO

Mediator Complex Regulates Alternative mRNA Processing via the Med23 Subunit

2012-02-24 | GSE33771 | GEO