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ABSTRACT: Gamma aminobutyric acid production by commercially available probiotic strains

PROVIDER: PRJNA875295 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR21418959.fastq.gz	Fastqsanger.gz
	SRR21418960.fastq.gz	Fastqsanger.gz
	SRR21418961.fastq.gz	Fastqsanger.gz
	SRR21418962.fastq.gz	Fastqsanger.gz
	SRR21418963.fastq.gz	Fastqsanger.gz

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In vitro fermentation model (Batch cultures)

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Priming hepatocytes into G2 phase reverses age-related regenerative impairments in livers of elderly mice

Project description:Frozen liver tissue from 24 month old C57BL/6 mice; control or each of the other three treatment groups taken pre and 36 hour post hepatectomy were used for analysis (n=2 per group). Mice were administrated intraperitoneally with one of the following agents every day for 7 days and 10 minutes before hepatectomy or hepatocyte isolation: 0.5 ml of saline, 500 umol alpha-aminobutyric acid or 500 umol gamma-aminobutyric acid in 0.5ml of saline. Mice in control group received no pre-administrations.

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Meta-transcriptomic Analyses Reveal Improved Gamma-amino butyric acid Production Machinery in Levilactobacillus brevis NPS-QW 145 Co-cultured with Streptococcus thermophilus ASCC1275 during Milk Fermentation

Project description:Purpose: High γ-aminobutyric acid (GABA)-producing Levilactobacillus brevis strain NPS-QW 145 along with Streptococcus thermophilus (one of the two starter bacteria used to make yogurt for its proteolytic activity) to enhance GABA production in milk. But a mechanistic understanding on how Levilactobacillus brevis cooperated with S. thermophilus to stimulate GABA production has been lacking. Method: Metatranscriptomic analyses combined with peptidomics were carried out to unravel the casein and lactose utilization patterns during milk fermentation with the co-culture. Results: We found particular peptides hydrolyzed by S. thermophilus 1275 were transported and biodegraded with peptidase in Lb. brevis 145 to meet the growth needs of the latter. In addition, amino acid synthesis and metabolism in Lb. brevis 145 were also activated to further support its growth. Glucose, as a result of lactose hydrolysis by S. thermophilus 1275, but not available lactose in milk, was outcompeted by Lb. brevis 145 as a main carbon source for glycolysis to produce ATP.In the stationary phase, under the acidic condition due to accumulation of lactic acid produced by S. thermophilus 1275, genes expression involved in pyridoxal phosphate (coenzyme of glutamic acid decarboxylase) metabolism and glutamic acid decarboxylase (Gad) in Lb. brevis 145 were induced for GABA production.

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Transcriptomics reveal strain-specific metabolic strategies for acid resistance and gamma-aminobutyric acid (GABA) production in Levilactobacillus brevis.

Project description:Transcriptomics reveal strain-specific metabolic strategies for acid resistance and gamma-aminobutyric acid (GABA) production in Levilactobacillus brevis.

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The impact of a consortium of fermented milk strains on the gut microbiome of gnotobiotic mice and monozygotic twins. (RNA-Seq)

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