Ontology highlight
ABSTRACT:
PROVIDER: PRJNA875594 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Novel environmentally safe and cost-efficient alternatives to pest management are needed. Seed priming enables plants to respond faster and more effectively to stress, and has emerged as a promising and sustainable tool. We previously demonstrated that seed priming with methyl jasmonate (MeJA) or salicylic acid (SA) enhances pest resistance without growth penalties in Arabidopsis thaliana. Here, we investigate whether these benefits extend to crops such as Brassica rapa, and whether the underlying molecular mechanisms are conserved. We compared the effects of MeJA and SA seed priming in A. thaliana and B. rapa. In both species, primed plants display enhanced resistance against the generalist mite Tetranychus urticae and the specialist caterpillar Pieris brassicae, without compromising plant growth. Transcriptomic and metabolomic profiling revealed that, despite broadly conserved co-expression network structures, the overlap in differentially expressed genes and metabolic shifts was limited. Additionally, the magnitude and direction of priming effects varied depending on both the plant and the herbivore, revealing strong plant-pest interaction effects. B. rapa responses–characterized by SA accumulation under mite infestation and glucosinolate pathway activation under caterpillar feeding–were robust, whereas A. thaliana displayed more flexibility. These divergent outcomes likely reflect epigenetic regulation and the complex genome architecture of B. rapa. Our findings suggest that the phenotypic benefits of seed priming are conserved across Brassicaceae, but their molecular basis are shaped by plant species and pest identity, as well as context-specific interactions. This comparative study underscores the potential of tailoring priming strategies to crop species for sustainable pest management.
2026-09-23 | GSE309717 | GEO
Project description:Seed defence priming is emerging as a novel, cost-efficient and environmentally safe tool for pest management. Previous reports suggest that this strategy could potentially uncouple the defence-growth trade-off in plants, but this role and the mechanisms underlying it remain elusive. Here, we investigated the ability of seed priming with four phytohormones and two plant vitamins to uncouple the growth-defence trade-off in Arabidopsis thaliana upon pest infestation, focusing on its molecular dynamics. To broaden our scope, two phytophagous species from different feeding guilds were used: the generalist cell-content sucker Tetranychus urticae, and the specialist chewer Pieris brassicae. A combined -omics approach revealed distinct and interactive effects of seed priming and herbivory. Specifically, seed priming with 0.1 mM methyl jasmonate induced a state of readiness characterized by reduced stomatal aperture, high transcriptomic variation, increased sugar content and enhanced translational rate. Upon pest infestation, primed plants exhibited herbivore-specific responses. Interaction of seed priming and T. urticae infestation increased amino acid levels, hydroxycinnamic acid amide production, and reallocated resources from arabidopsides and anthocyanin biosynthetic pathways. In contrast, P. brassicae infestation in primed plants led to enhanced anthocyanin biosynthesis, alterations in lignin metabolic intermediates, and sustained carbon and nitrogen sources. These findings demonstrate that seed priming can enhance pest-specific defences in plants without incurring a fitness penalty, effectively managing infestations while maintaining growth.
2025-09-18 | GSE287464 | GEO
Project description:we investigated the effect of exposing 4 successive generations of Arabidopsis thaliana to severe heatwave conditions. We show that heat-primed offspring has increased survival rates and seed production after exposure to heat wave conditions. Bisulfite sequencing allowed us to link this to altered DNA methylation, including a global hypomethylation of the mitochondrial DNA. mRNA sequencing showed that priming affected genes related to the mitochondrial energy system and oxidative stress responses.
2025-06-01 | GSE233248 | GEO
Project description:we investigated the effect of exposing 4 successive generations of Arabidopsis thaliana to severe heatwave conditions. We show that heat-primed offspring has increased survival rates and seed production after exposure to heat wave conditions. Bisulfite sequencing allowed us to link this to altered DNA methylation, including a global hypomethylation of the mitochondrial DNA. mRNA sequencing showed that priming affected genes related to the mitochondrial energy system and oxidative stress responses.
2025-06-01 | GSE233247 | GEO
Project description:gnp3_tri33-arabidoseed. Embryo seed development. WP3: Biodiversity of seed traits: state-of-the-art. Analysis of the expression of small RNAs precursors in the seed development of Arabidopsis thaliana.
2011-04-22 | GSE28311 | GEO
Project description:Plant volatiles can mediate plant-plant communication in the sense that plants attacked by herbivores can signal their unattacked neighbors of danger by emitting HIPVs. We call this the priming effect. Since the plant defense response is a systematic process involving numerous pathways and genes,to characterize the priming process, a time course study using a genome-wide microarray may provide more accurate information about the priming process. Furthermore, to what extent do the priming process and direct defense share similar gene expression profiles or pathways are also not clear. We used microarray to detect the priming effect of plant volatiles to healthy Arabidopsis thaliana, and the effect of direct leafminer feeding to Arabidopsis thalianas. A system using Lima bean plants, from which HIPVs can be effectively induced by leafminer feeding, as emitters and Arabidopsis thaliana as receivers is used to track the priming process between neighbor plants. The Arabisopsis thaliana seedlings were treated by volatiles from leafminer fed lima bean for 24h or 48h for RNA extraction and hybridization on Affymetrix microarrays. The Arabisopsis thaliana seedlings fed by leafminer directly were also collected The for RNA extraction and hybridization on Affymetrix micorarrays. We want to explore the response of Arabidopsis thaliana to priming volatiles during a 24h-48h time course. We also want to compare the effect of priming and direct leafminer feeding.
2013-09-24 | E-GEOD-33505 | biostudies-arrayexpress
Project description:Untargeted metabolomic analyses were carried out on seed coat/endosperm and seed embryo (dry seeds) of Arabidopsis thaliana Columbia-0 genotype. Three biological replicates were analyzed for each sample.
| MSV000095844 | MassIVE
Project description:We report the dry seed transcriptome of 13 wild type accessions of Arabidopsis thaliana
2022-06-06 | GSE179008 | GEO
Project description:In order to identify differentially expressed genes in developing seeds of Arabidopsis thaliana three different stages of seed development were analysed (9-10, 10-11 and 12-13 days after flower opening) for two Arabidopsis thaliana accessions, Col-0 and C24. For each stage and accession three biological replicates were analysed.
2017-05-21 | GSE94763 | GEO
Project description:In order to identify differentially expressed genes in developing seeds of Arabidopsis thaliana three different stages of seed development were analysed (9-10, 10-11 and 12-13 days after flower opening) for two Arabidopsis thaliana accessions, Col-0 and C24. For each stage and accession three biological replicates were analysed.
2017-05-21 | GSE94762 | GEO