Project description:Transcription profiling by array of 10 days old Brassica rapa ssp. chinensis seedlings treated with 2mM methyl jasmonate by spraying and harvesting 48 hours past treatment
Project description:Root and leaf samples from Brassica rapa line R-O-18 were compared. The results will be compared to the same samples hybridised to the Affymetrix Brassica Exon 1.0 ST array. 6 samples were hybridised. Triplcate samples of 11 day old roots and 2 semi-expanded leaves from 23 day old Brassica rapoa R-O-18 plants.
Project description:Purpose: we used transcriptional profiling to identify differentially expressed genes in Col-0 and OE-SAUR41 seedlings Methods: Total RNA of 5-day-old Arabidopsis roots and the shoot parts were send to Vazyme BIotech Co., Ltd (Nanjing, China) to carry out library construction, Illumina HiSeq sequencing, and bioinformatical analysis. Each sample generated 4 Gb of clean data and contained three biological replicates.
Project description:Purpose: we used transcriptional profiling to identify differentially expressed genes in Col-0 and saur41/4071/72 seedlings Methods: Total RNA of 5-day-old Arabidopsis roots and the shoot parts were send to Vazyme BIotech Co., Ltd (Nanjing, China) to carry out library construction, Illumina HiSeq sequencing, and bioinformatical analysis. Each sample generated 4 Gb of clean data and contained three biological replicates.
Project description:Auxin amino acid conjugates are considered storage forms of auxins available as a source of active auxins on the plant demand. We treated Brassica rapa seedlings with 0.01 mM indole-3-acetyl-L-alanine (IAA-Ala), indole-3-propionyl-L-alanine (IPA-Ala), and indole- 3-butyryl-L-alanine (IBA-Ala) and examined their effects on the transcriptome. All auxin conjugate treatments caused similar patterns in transcription profiles compared to the control, but with different intensities of over- and under-expression depending on the treatment. Most auxin-related DEGs were identified after IBA-Ala treatment, followed by IPA-Ala and IAA-Ala, respectively.
Project description:In this study genome-wide gene expression profiling was used to analyze mechanisms of drought tolerance in Brassica rapa. Using an Illumina Mi-Seq platform we sequenced RNA from shoot tissues of drought tolerant and drought sensitive B. rapa genotypes in control conditions and after application of osmotic stress. Differentially expressed genes between the different conditions and genotypes were used to identify drought relevant gene networks.