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Genome-wide comparative methylation analysis reveals the fate of germ stem cells after surrogate production in teleost

ABSTRACT: Genome-wide comparative methylation analysis reveals the fate of germ stem cells after surrogate production in teleost

PROVIDER: PRJNA877682 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR21470603_1.fastq.gz	Fastqsanger.gz
	SRR21470603_2.fastq.gz	Fastqsanger.gz
	SRR21470605_1.fastq.gz	Fastqsanger.gz
	SRR21470605_2.fastq.gz	Fastqsanger.gz
	SRR21470607_1.fastq.gz	Fastqsanger.gz

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Genome-wide comparative methylation analysis reveals the fate of germ stem cells after surrogate production in teleost

Project description:Surrogate production technologies in fish by germ stem cell transplantation into the sterile recipient is becoming popular but lacks epigenetic information of the donor-derived gametes. In our study, we have thoroughly investigated and compared the genome-wide DNA methylation pattern of donor-derived gametes, and the progeny derived from them in two different types of chimera (interspecific and intraspecific) with the donor strain.

2024-02-07 | GSE212876 | GEO

Genome-wide comparative methylation analysis reveals the fate of germ stem cells after surrogate production in teleost.

Project description:BackgroundSurrogate production by germline stem cell transplantation is a powerful method to produce donor-derived gametes via a host, a practice known as surrogacy. The gametes produced by surrogates are often analysed on the basis of their morphology and species-specific genotyping, which enables conclusion to be drawn about the donor's characteristics. However, in-depth information, such as data on epigenetic changes, is rarely acquired. Germ cells develop in close contact with supporting somatic cells during gametogenesis in vertebrates, and we hypothesize that the recipient's gonadal environment may cause epigenetic changes in produced gametes and progeny. Here, we extensively characterize the DNA methylome of donor-derived sperm and their intergenerational effects in both inter- and intraspecific surrogates.ResultsWe found more than 3000 differentially methylated regions in both the sperm and progeny derived from inter- and intraspecific surrogates. Hypermethylation in the promoter regions of the protocadherin gamma gene in the intraspecific surrogates was found to be associated with germline transmission. On the contrary, gene expression level and the embryonic development of the offspring remained unaffected. We also discovered MAPK/p53 pathway disruption in interspecific surrogates due to promoter hypermethylation and identified that the inefficient removal of meiotic-arrested endogenous germ cells in hybrid gonads led to the production of infertile spermatozoa.ConclusionsDonor-derived sperm and progeny from inter- and intraspecific surrogates were more globally hypermethylated than those of the donors. The observed changes in DNA methylation marks in the surrogates had no significant phenotypic effects in the offspring.

| S-EPMC10870548 | biostudies-literature

Teleost Species

Project description:Teleost Fish species

| PRJNA1297367 | ENA

Teleost Species

Project description:Teleost Fish species Other

| PRJNA1294866 | ENA

Distinct mechanisms of germ cell factor regulation for an inductive germ cell fate

Project description:Distinct mechanisms of germ cell factor regulation for an inductive germ cell fate

| PRJNA806693 | ENA

rbm24a is an organizer component of germ plasm to determine germ cell fate

Project description:rbm24a is an organizer component of germ plasm to determine germ cell fate

| PRJNA1109723 | ENA

rbm24a is an organizer component of germ plasm to determine germ cell fate

Project description:rbm24a is an organizer component of germ plasm to determine germ cell fate

| PRJNA1111448 | ENA

IGF2BP1 stabilizes OTX2 mRNA in an m6A-dependent manner to restrict human germ cell fate by repressing TFAP2C

Project description:N6-methyladenosine (m6A) is the most abundant and reversible modification on messenger RNAs. The differential m6A profiles in different cell types are installed by writers (such as METTL3) and removed by erasers (such as FTO). The m6A-modified RNAs are recognized by readers to trigger downstream regulation of RNA metabolism, eventually contributing to the regulation of cell fates. Primordial germ cells (PGCs) are specified early during embryogenesis and establish the germ cell lineage for transmitting genetic and epigenetic information from parents to offspring. Defects in the establishment of germline may lead to infertility, germ cell tumors, and birth defects. Despite extensive research, the m6A-mediated epigenetic regulation of the specification of PGCs remains elusive. In this study, we discovered that knock-out of m6A writer or over-expression of erasers leads to increased percentage of human PGC-like cells (hPGCLCs) induced from embryonic stem cells. We identified the m6A reader IGF2BP1 as the key factor for restricting hPGCLC fate induction by stabilizing OTX2 mRNAs in an m6A-dependent manner. Moreover, we discovered that OTX2 suppresses TFAP2C during germ cell lineage specification. In summary, we characterized the m6A-IGF2BP1-OTX2-TFAP2C axis in restricting the specification of human germ cell fate.

| PXD051996 |

rbm24a is an organizer component of germ plasm to determine germ cell fate (202409 validation)

Project description:rbm24a is an organizer component of germ plasm to determine germ cell fate (202409 validation)

| PRJNA1216205 | ENA

Teleost fish

Project description:Transcriptomic evolution of the olfactory organ in teleost fishes

| PRJNA899054 | ENA