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Analysis of tumor cell plasticity in endometrioid carcinoma using cancer stem cell marker aldehyde dehydrogenase (ALDH)

ABSTRACT: Analysis of tumor cell plasticity in endometrioid carcinoma using cancer stem cell marker aldehyde dehydrogenase (ALDH)

PROVIDER: PRJNA877768 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR21474390.fastq.gz	Fastqsanger.gz
	SRR21474391.fastq.gz	Fastqsanger.gz
	SRR21474392.fastq.gz	Fastqsanger.gz
	SRR21474393.fastq.gz	Fastqsanger.gz
	SRR21474394.fastq.gz	Fastqsanger.gz

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Analysis of tumor cell plasticity in endometrioid carcinoma using cancer stem cell marker aldehyde dehydrogenase (ALDH)

Project description:To investigate the tumor cell plasticity using aldehyde dehydrogenase (ALDH) 1A1 activity, we established two distinct HEC-1B sublines, in which ALDH1A1-high population accelerated such transition and ALDH1A1-high population did not show such acceleration.

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Gene expression data from endometrial cancer patient-derived cells (PDCs) treated with aldehyde dehydrogenase inhibitor

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Progenitor Cell Marker Aldehyde Dehydrogenase 1a3 Defines a Subset of Failing Pancreatic Beta Cells in Diabetic Mice

Project description:Insulin-producing beta cells become dedifferentiated during diabetes progression. An impaired ability to select substrates for oxidative phosphorylation, or metabolic inflexibility, sets the stage for progression from beta cell dysfunction to beta cell dedifferentiation. In this study, we sought to isolate and functionally characterize failing beta cells, as a preliminary step to identify pathways to reverse dedifferentiation. Using various experimental models of diabetes, we found a striking enrichment in the expression of aldehyde dehydrogenase 1 isoform A3 (ALDH+) as beta cells become dedifferentiated. Flow-sorted ALDH+ islet cells demonstrate impaired glucose-induced insulin secretion, are depleted of Foxo1 and MafA, and include a Neurogenin3-positive subset. RNA sequencing analysis demonstrates that ALDH+ cells are characterized by: (i) impaired oxidative phosphorylation and mitochondrial complex I, IV, and V; (ii) activated RICTOR; and (iii) progenitor cell markers. We propose that impaired mitochondrial function marks the progression from metabolic inflexibility to dedifferentiation in the natural history of beta cell failure. RNA-Sequencing analysis of 2 different cell types in 2 different genotype categories.

2016-09-02 | E-GEOD-78966 | biostudies-arrayexpress

Progenitor Cell Marker Aldehyde Dehydrogenase 1a3 Defines a Subset of Failing Pancreatic Beta Cells in Diabetic Mice

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Impact of Aldehyde Dehydrogenase Inhibition on the Stemness of Endometrial Cancer Stem Cells

Project description:Cancer stem cells (CSCs) are crucial for tumor initiation, growth, dissemination, and resistance to therapy. In this study, we aimed to evaluate the effect of inhibiting aldehyde dehydrogenase (ALDH), an enzyme present in endometrial CSCs, using N,N-diethylaminobenzaldehyde (DEAB). This study aimed to evaluate the effects of ALDH inhibition in endometrial cancer stem cells (CSCs). ECC-1 and RL95-2 cell lines were subjected to proteome profiling with and without N,N-diethylaminobenzaldehyde (DEAB).

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Project description:Clostridium ljungdahlii not only utilizes CO, but also H2 as energy source during autotrophic growth. And C. ljungdahlii also grows in fructose fermentation. In theory, fructose is a more energetically favourable energy source than syngas in the fermentation of C. ljungdahlii. However, C. ljungdahlii grows insufficiently in fructose and produces less acetate and ethanol, compared to syngas fermentation. In this study, C. ljungdahlii wild type and mutants were fermented on fructose. C. ljungdahlii produced less ethanol than the ΔadhE1 mutant and consumed less fructose. The ΔadhE1+2 mutant cannot grow in the syngas fermentation and produced less ethanol among the three strains. The results showed that aldehyde dehydrogenase inactivation led to efficient metabolism in C. ljungdahlii and the bifunctional aldehyde/alcohol dehydrogenases inactivation led to decrease metabolism. Thus, comparative transcriptomes among cells grown on fructose of three strains were performed to investigate gene expression profiles based on three biological replicates.

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