Project description:The Illumina Infinium MethylationEPIC Beadchip was used to obtain genomewide methylation profiles from Human Urine Renal Progenitors obtained after kidney transplantation from lived donors or dead donors
Project description:Kidney transplantation is the preferred treatment for kidney failure, offering improved survival, quality of life and cost-effectiveness compared to dialysis. However, post-transplant management is challenging due to the limited lifespan of transplanted organs, often requiring repeat transplants. Current methods for monitoring post-transplant complications are invasive and have limitations. Therefore, there is an urgent need for novel non-invasive biomarkers. This study investigates the proteomic composition of full urine as a source of information to understand renal biology during the process of transplantation and to identify potential markers for outcome prediction. Urine samples were collected from donors before transplantation and from recipients 4 weeks and 1 year after transplantation. Proteomic analysis was performed using mass spectrometry and label-free quantification. This study underscores the potential of non-invasive urine proteomics for identifying biological processes involved in the response of a kidney to transplantation and for enhancing post-transplant monitoring and outcome prediction.
Project description:Kidney transplantation is the preferred treatment for kidney failure, offering improved survival, quality of life and cost-effectiveness compared to dialysis. However, post-transplant management is challenging due to the limited lifespan of transplanted organs, often requiring repeat transplants. Current methods for monitoring post-transplant complications are invasive and have limitations. Therefore, there is an urgent need for novel non-invasive biomarkers. This study investigates the proteomic composition of full urine as a source of information to understand renal biology during the process of transplantation and to identify potential markers for outcome prediction. Urine samples were collected from donors (timepoint A) before transplantation and from recipients 4 weeks (timepoint B) and 1 year (timepoint C) after transplantation. Proteomic analysis was performed using mass spectrometry and label-free quantification. This study underscores the potential of non-invasive urine proteomics for identifying biological processes involved in the response of a kidney to transplantation and for enhancing post-transplant monitoring and outcome prediction.
Project description:Kidney transplantation is the treatment of choice for patients with end-stage chronic kidney disease (ESKD). Despite the usefulness of transplantation as replacement therapy, long-term graft survival represents a major challenge for transplant immunology. Although nowadays there has been an advance in understanding immunological mechanisms mediating rejection, and the improvement of immunomodulation therapies, there are still underlying molecular processes marking an important variability among patients, and presumably influencing allograft rejection. With our analysis we explored differences in gene expression by Next Generation Sequencing implementing RNA-Seq in biopsies, blood and urine from kidney transplant patients with acute and chronic rejection. For this, we performed an intra-outcome analysis simultaneously in acute and chronic rejection, with which we sought: 1. To identify differences in gene expression between peripheral blood vs renal tissue and peripheral blood vs urine in acute rejection and chronic rejection; 2. To identify the level of agreement in gene expression between renal tissue and urine in acute rejection and chronic rejection and 3. To identify genes and biological processes associated with acute rejection and chronic rejection that could be potentially detected in blood, and simultaneously in urine and biopsy in acute rejection and in chronic rejection.
Project description:We characterized the transcriptome and methylome of renal progenitor cells in the urine of a cohort of stable kidney transplant recipients to elucidate whether the origin of the donor (living or deceased donor) was associated with a specific molecular reprogramming.
Project description:This study represents the first quantitative analysis of the temporal changes in the small urinary extracellular vesicle proteome throughout living donor kidney transplantation identifying PCK2 abundance as a biomarker for renal function 12 months after transplantation
Project description:Kidney damage involves the progressive and inexorable destruction of tubular and glomerular system. However, it is known that the patients survive AKI often recover renal structure and function. Correspondingly, previous studies demonstrated tubular regeneration in mice after massive kidney injury and linked mouse Sox9+ renal progenitor cells to this process. Here we show that progenitor cells can be cloned from mouse medulla and cortex. Clones can be grown from a single cell and indefinitely passaged. Progenitor cells derived from renal medulla can readily assembly into “kidney organoids” expressing proximal/distal tubular cell markers in 3D culture.