Project description:Monkeypox virus: Genome sequencing by Virus National Reference Laboratory, Center for Virology Medical University of Vienna, Vienna, Austria
Project description:The discovery of noncoding RNAs is reshaping paradigms in molecular biology and virology. Circular RNAs (circRNAs), a class of novel noncoding RNAs, their circular conformation endows these transcripts with critical physiological and pathological roles. Here, we employed circRNAs sequencing technologies, following RNase R treatment on influenza virus-infected human pulmonary epithelial cells to identify and characterize influenza virus-derived circRNAs. Back spicing junctions mapping analysis identified lots of circRNAs from 7 viral gene segment apart from M gene and subsequent experiments confirmed the presence of these IAV-encoded circRNAs in infected cells and mice lung. We found that polymerase genes could encode numerous viral circRNA (vcircRNAs) within infected cells, such as NP and PB1. By vcircRNA overexpression and knockdown with small interfering RNA, we found that these vcircRNAs is required for efficient viral replication. Notably, IAV-derived circRNAs biogenesis was formed with canonical GU/AG splicing mechanism in infected A549 cells. Splicing site mutant viruses displayed a significant decrease in the viral replication in vitro and pathogenicity in mice. Overall, our findings will not only uncover the novel RNA transcripts in IAVs infection cycle, but also provide a new perspective to investigate the crosstalk between host and IAVs from vcircRNAs.
Project description:Influenza virus infection leads to global cardiac proteome remodeling during convalescence MTD project_description "Influenza virus infections lead to more than 500,000 hospitalizations in the U.S. every year. Patients with cardiovascular diseases have been shown to be at high risk of influenza mediated cardiac complications. Importantly, recent reports have provided clinical data supporting a direct link between laboratory-confirmed influenza virus infection and adverse cardiac events. However, the molecular mechanisms of how influenza virus infection induces detrimental cardiac changes, even after resolution of the pulmonary infection, is completely unknown.
Project description:Influenza virus infection leads to global cardiac proteome remodeling during convalescence MTD project_description "Influenza virus infections lead to more than 500,000 hospitalizations in the U.S. every year. Patients with cardiovascular diseases have been shown to be at high risk of influenza mediated cardiac complications. Importantly, recent reports have provided clinical data supporting a direct link between laboratory-confirmed influenza virus infection and adverse cardiac events. However, the molecular mechanisms of how influenza virus infection induces detrimental cardiac changes, even after resolution of the pulmonary infection, is completely unknown. We performed global quantitative proteomics as well as phosphoproteomics in this study.
