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PbAP2-FG2 and AP2R-2 function together as a transcriptional repressor complex essential for Plasmodium female development

ABSTRACT: PbAP2-FG2 and AP2R-2 function together as a transcriptional repressor complex essential for Plasmodium female development

PROVIDER: PRJNA882421 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR21640351.fastq.gz	Fastqsanger.gz
	SRR21640352.fastq.gz	Fastqsanger.gz
	SRR21640353.fastq.gz	Fastqsanger.gz
	SRR21640354.fastq.gz	Fastqsanger.gz
	SRR21640355.fastq.gz	Fastqsanger.gz

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PbAP2-FG2 and AP2R-2 function together as a transcriptional repressor complex essential for Plasmodium female development [RNA-seq]

Project description:Plasmodium berghei transcriptomes of pbap2-fg2 and ap2r-2 knockout parasites [pbap2-fg2(-) and ap2r-2(-), respectively] were analyzed at the gametocyte stage.

2023-02-02 | GSE213773 | GEO

PbAP2-FG2 and AP2R-2 function together as a transcriptional repressor complex essential for Plasmodium female development [ChIP-seq]

Project description:To investigate the genome-wide binding sites of the transcriptional regulators, PbAP2-FG2 and AP2R-2 in female gametocytes of Plasmodium berghei, the chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq) analyses were performed. Parasites expressing GFP-fused PbAP2-FG2 (PbAP2-FG2::GFP) or GFP-fused AP2R-2 (AP2R-2::GFP) were harvested after treating the infected mice with sulfadiazine and subjected to ChIP-seq experiments using anti-GFP antibody. Binding sites of PbAP2-FG2 and AP2R-2 were determined from the sequence data.

2023-02-02 | GSE213775 | GEO

PbAP2-FG2 and AP2R-2 function together as a transcriptional repressor complex essential for Plasmodium female development [RNA-seq]

Project description:PbAP2-FG2 and AP2R-2 function together as a transcriptional repressor complex essential for Plasmodium female development [RNA-seq]

| PRJNA882419 | ENA

PbAP2-FG2 and AP2R-2 function together as a transcriptional repressor complex essential for Plasmodium female development [ChIP-seq]

Project description:PbAP2-FG2 and AP2R-2 function together as a transcriptional repressor complex essential for Plasmodium female development [ChIP-seq]

| PRJNA882428 | ENA

PbAP2-FG2 and PbAP2R-2 function together as a transcriptional repressor complex essential for Plasmodium female development.

Project description: Not available

| S-EPMC9956629 | biostudies-literature

PbAP2-TR plays a role in regulating major transcriptomic changes from early to late trophozoite [pbap2-g_KO_RNA-seq]

Project description:Plasmodium berghei transcriptomes of gametocyte-absent mutant parasites, the pbap2-g-knockout line (pbap2-g(-)), were analyzed from ring to trophozoite stages by high-throughput RNA sequencing (RNA-seq) analysis.

2025-07-01 | GSE290541 | GEO

PbAP2-TR plays a role in regulating major transcriptomic changes from early to late trophozoite [PbAP2-TR_RNA-seq]

Project description:Plasmodium berghei transcriptomes of pbap2-tr-DiCre, a parasite line in which pbap2-tr is disrupted by DiCre-mediated recombination in the presence of rapamycin, were analyzed at the trophozoite stage.

2025-07-01 | GSE290545 | GEO

PbAP2-FG2 and AP2R-2 function together as a transcriptional repressor complex essential for Plasmodium female development

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-02-02 | GSE213776 | GEO

PbAP2-TR plays a role in regulating major transcriptomic changes from early to late trophozoite [PbAP2-TR_ChIP-seq]

Project description:To investigate the genome-wide binding sites of PbAP2-TR and PbMORC in the asexual blood stage of Plasmodium berghei, the chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq) analyses were performed. Parasites expressing GFP-fused PbAP2-TR or GFP-fused PbMORC were generated from the Cas9-expressing parasite (PbCas9), and pbap2-g was disrupted in these parasites (PbAP2-TR::GFPpbap2-g(-) and PbMORC::GFPpbap2-g(-)). Whole blood was harvested from mice infected with these transgenic parasites at 12 h post infection,and the parasites were then subjected to ChIP-seq experiments using anti-GFP antibody. Genome-wide binding sites of PbAP2-TR were determined from the sequence data.

2025-07-01 | GSE290542 | GEO

Plasmodium transcription repressor AP2-O3 regulates sex-specific identity of gene expression in female gametocytes [RNA-seq]

Project description:Male and female gametocytes are sexual precursor cells essential for transmission of malaria parasite in the mosquitoes. Differentiation of gametocytes to fertile gametes (gametogenesis) relies on the gender-specific transcriptome. However, how the parasites establish distinct repertoire of gene transcription in the male and female gametocytes remains largely unknown. Here, we report that an Apetala2 (AP2) family transcription factor (TF) AP2-O3 operates as a transcription repressor regulating female gametocyte transcriptome. AP2-O3 is specifically localized in the nucleus of the female gametocytes. AP2-O3-deficient parasites produce apparently normal female gametocytes, which fail to differentiate to fully fertile female gametes, leading to developmental arrest in fertilization and early development post-fertilization. AP2-O3 disruption causes massive up-regulation of transcriptionally dormant male genes and simultaneously down-regulation of highly transcribed female genes in female gametocytes. ChIP-seq and EMSA analysis establish AP2-O3 as a transcription repressor that targets a significant proportion of the upregulated male genes by recognizing an eight-base DNA motif in the promoters. In addition, the maternal AP2-O3 is removed after fertilization, which is required for the zygote to ookinete development. These results demonstrate that global transcriptional repression of male genes in the female gametocytes is required for safeguarding female-specific transcriptome and essential for the mosquito transmission of Plasmodium.

2021-01-26 | GSE157456 | GEO

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