Ontology highlight
ABSTRACT:
PROVIDER: PRJNA882568 | ENA |
REPOSITORIES: ENA
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Project description:Sequencing of 16S ribosomal RNA (rRNA) gene, which has improved the characterization of microbial community, has made it possible to detect a low level Helicobacter pylori (HP) sequences even in HP-negative subjects which were determined by a combination of conventional methods. This study was conducted to obtain a cutoff value for HP colonization in gastric mucosa biopsies and gastric juices by the pyrosequencing method. Corresponding author: Department of Internal Medicine, Seoul National University Bundang Hospital, Seoungnam, Gyeonggi-do, Korea; Department of Internal Medicine and Liver Research Institute, Seoul National University College of Medicine, Seoul, Korea (Tel., +82-31-787-7008; e-mail, nayoungkim49@empas.com). Microbial DNA from gastric mucosal samples [gastric antrum (n=63, mucosal biopsy), follow-up sample on gastric antrum (n=16, mucosal biopsy), and gastric body (n=18, mucosal biopsy)] and gastric juices (n=4, not mucosal biopsy) was amplified by nested PCR using universal bacterial primers, and the 16S rRNA genes were pyrosequenced.
2014-09-18 | E-GEOD-61493 | biostudies-arrayexpress
Project description:Focal and diffuse damage to the gastric mucosa contributes to gastric mucosal injury (GMI). Dietary fiber may exert protective effects against GMI; however, its use as a gastrointestinal intervention remains limited. Here, we assessed the potential role of dietary fiber Riclin in GMI. Dietary fiber Riclin exerted a dual effect in enhancing gastric mucosal defense. It modulated microbial-metabolite composition, enhancing biological barriers and reinforcing sustained adhesion to the gastric mucus bicarbonate barrier. Additionally, Riclin enhanced the gastric mucosal immune barrier through the NOD/TLR4/NF-κB pathway. The protective role of Riclin in GMI was revealed by constructing an alcohol-induced murine gastric ulcer model. Our results propose Riclin as a novel dietary fiber with the potential to ameliorate a wide range of gastric disorders characterized by a disrupted gastric mucosal defense and immune dysregulation. In particular, we underscore its potential as a promising dietary fiber for promoting gastric health.
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Project description:To explore the role of miRNAs in gastric cancer, miRNA microarray profiling in 28 pairs of gastric cancer tissues and the matched normal mucosal tissues were performed. Twenty-eight pairs of gastric cancer tissues and the matched normal mucosal tissues were performed by human microRNA microarray v.12.0 (Agilent Technologies).
2016-09-08 | E-GEOD-78775 | biostudies-arrayexpress
Project description:To explore the role of miRNAs in gastric cancer, miRNA microarray profiling in 28 pairs of gastric cancer tissues and the matched normal mucosal tissues were performed.
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Project description:Persistent mucosal inflammation and microbial infection are characteristic of Chronic Rhinosinusitis (CRS). Though mucosal microbiota dysbiosis is a characteristic feature of other chronic inflammatory diseases, the relationship between sinus microbiota composition and CRS is unknown. Here we demonstrate, using comparative microbiome profiling of a cohort of CRS patients and healthy subjects, that the sinus microbiota of CRS patients exhibit significantly reduced bacterial diversity. Characteristic of this community collapse is the depletion of multiple, phylogenetically distinct, Lactic Acid Bacteria and the concomitant increase in relative abundance of a single species, Corynebacterium tuberculostearicum. Recapitulating the conditions observed in our human cohort in a murine model confirmed the pathogenic potential of C. tuberculostearicum and the critical necessity for a replete mucosal microbiota to protect against this species. Moreover, we provide evidence that Lactobacillus sakei, identified from our comparative microbiome analyses as a potentially protective species, affords defense against C. tuberculostearicum sinus infection, even in the context of a depleted sinus bacterial community. These studies demonstrate that sinus mucosal health is highly dependent on the composition of the resident microbiota, and identifies a new sino-pathogen and a strong bacterial candidate for therapeutic intervention. A total of 14 samples were profiled for microbiome composition: 7 from non-sinusitis patients, and 7 from patients with clinically diagnosed chronic sinusitis.
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Project description:Even after endoscopic treatment of early gastric adenocarcinoma (GAC) and eradication of Helicobacter pylori (H. pylori), some patients develop a metachronous recurrence (MR), the mechanism of which is still unknown. To elucidate the mechanism and risk factors for MR, we analyzed gene expression at multiple locations of the gastric mucosa, considering the heterogeneity of gastric mucosal damage caused by H. pylori infection and investigated the mechanism and risk factors for MR.
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