Dataset Information

Gene regulation in activated microglia by adenosine A3 receptor agonists. A transcriptomics analysis.

ABSTRACT: Gene regulation in activated microglia by adenosine A3 receptor agonists. A transcriptomics analysis.

PROVIDER: PRJNA885011 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	SRR21736357_1.fastq.gz	Fastqsanger.gz
	SRR21736357_2.fastq.gz	Fastqsanger.gz
	SRR21736358_1.fastq.gz	Fastqsanger.gz
	SRR21736358_2.fastq.gz	Fastqsanger.gz
	SRR21736359_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 14

Similar Datasets

Project description:The A3 adenosine receptor (AR) is an important inflammatory and immunological target. However, the underlying mecha-nisms are not fully understood. Here, we report the gene regulation in HL-60 cells treated acutely with highly selective A3AR agonist MRS5698, positive allosteric modulator (PAM) LUF6000, or both. Both pro- and anti-inflammatory genes, such as IL-1a, IL-1β, and NFκBIZ, are significantly upregulated. During our observations, LUF6000 alone produced a lesser effect, while the MRS5698 + LUF6000 group demonstrated generally greater effects than MRS5698 alone, consis-tent with allosteric enhancement. The number of genes up- and down-regulated are similar. Pathway analysis highlighted the critical involvement of signaling molecules, including IL-6 and IL-17. Important upstream regulators include IL-1a, IL-1β, TNF-α, NF-κB, etc. PPAR, which modulates eicosanoid metabolism, was highly downregulated by the A3AR ago-nist. Considering previous pharmacological results and mathematical modeling, LUF6000’s small enhancement of genetic upregulation suggested that MRS5698 is a nearly full agonist, which we demonstrated in both cAMP and calcium assays. The smaller effect of LUF6000 on MRS5698 in comparison to its effect on Cl-IB-MECA was shown in both HL-60 cells endogenously expressing the human (h) A3AR and in recombinant hA3AR-expressing CHO cells, consistent with its HL-60 cell genetic regulation patterns. In summary, by using both selective agonists and PAM, we identified genes that are closely relevant to immunity and inflammation to be regulated by A3AR in differentiated HL-60 cells, a cell model of neutrophil function. In addition, we demonstrated the previously uncharacterized allosteric signaling-enhancing effect of LUF6000 in cells endogenously expressing the hA3AR. (PDF) Genetic and functional modulation by agonist MRS5698 and allosteric enhancer LUF6000 at the native A3 adenosine receptor in HL-60 cells. Available from: https://www.researchgate.net/publication/378553124_Genetic_and_functional_modulation_by_agonist_MRS5698_and_allosteric_enhancer_LUF6000_at_the_native_A3_adenosine_receptor_in_HL-60_cells#fullTextFileContent [accessed Jul 07 2025].

Dataset Information

Gene regulation in activated microglia by adenosine A3 receptor agonists. A transcriptomics analysis.

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets