Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA Polymerase II pausing temporally coordinates cell cycle progression and erythroid differentiation [NET-seq]

ABSTRACT: RNA Polymerase II pausing temporally coordinates cell cycle progression and erythroid differentiation [NET-seq]

PROVIDER: PRJNA885861 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR21771643.fastq.gz	Fastqsanger.gz
	SRR21771644.fastq.gz	Fastqsanger.gz
	SRR21771645.fastq.gz	Fastqsanger.gz
	SRR21771646.fastq.gz	Fastqsanger.gz
	SRR21771647.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 41

Similar Datasets

RNA Polymerase II pausing temporally coordinates cell cycle progression and erythroid differentiation

Project description:RNA Polymerase II pausing temporally coordinates cell cycle progression and erythroid differentiation

| PRJNA885855 | ENA

RNA Polymerase II pausing temporally coordinates cell cycle progression and erythroid differentiation [NET-seq]

Project description:The controlled release of promoter-proximal RNA polymerase II (Pol II) pausing into productive elongation is a major step in gene regulation. Yet, functionally analyzing Pol II pausing is difficult because the factors that regulate pause release are generally essential. Here, we identified heterozygous loss-of-function mutations in SUPT5H, encoding SPT5, in individuals with β-thalassemia trait unlinked to the β-globin locus. Recapitulating the pathogenic mutations in human erythroid cells or acute treatment with transcription inhibitors replicates the patient phenotype of altered globin gene expression. Furthermore, in SUPT5H-edited cells, Pol II pause release was globally disrupted. We observed dynamic patterns of pausing at cell cycle and erythroid differentiation genes at the transition from progenitors to precursors during erythropoiesis. At this same transition, in SUPT5H-edited cells, we observed delayed differentiation and altered cell cycle kinetics, as well as a delay in the onset of gene expression programs. Therefore, hindering pause release perturbs cell cycle and delays differentiation at key stages of erythropoiesis, revealing a role for Pol II pausing in the temporal coordination between the cell cycle and differentiation.

2023-07-21 | GSE214557 | GEO

RNA Polymerase II pausing temporally coordinates cell cycle progression and erythroid differentiation [CRISPR]

Project description:RNA Polymerase II pausing temporally coordinates cell cycle progression and erythroid differentiation [CRISPR]

| PRJNA885860 | ENA

RNA Polymerase II pausing temporally coordinates cell cycle progression and erythroid differentiation [RNA-seq]

Project description:RNA Polymerase II pausing temporally coordinates cell cycle progression and erythroid differentiation [RNA-seq]

| PRJNA885863 | ENA

RNA Polymerase II pausing temporally coordinates cell cycle progression and erythroid differentiation [CRISPR]

2023-07-21 | GSE214556 | GEO

RNA Polymerase II pausing temporally coordinates cell cycle progression and erythroid differentiation [RNA-seq]

2023-07-21 | GSE214558 | GEO

RNA polymerase II pausing temporally coordinates cell cycle progression and erythroid differentiation.

Project description:Controlled release of promoter-proximal paused RNA polymerase II (RNA Pol II) is crucial for gene regulation. However, studying RNA Pol II pausing is challenging, as pause-release factors are almost all essential. In this study, we identified heterozygous loss-of-function mutations in SUPT5H, which encodes SPT5, in individuals with β-thalassemia. During erythropoiesis in healthy human cells, cell cycle genes were highly paused as cells transition from progenitors to precursors. When the pathogenic mutations were recapitulated by SUPT5H editing, RNA Pol II pause release was globally disrupted, and as cells began transitioning from progenitors to precursors, differentiation was delayed, accompanied by a transient lag in erythroid-specific gene expression and cell cycle kinetics. Despite this delay, cells terminally differentiate, and cell cycle phase distributions normalize. Therefore, hindering pause release perturbs proliferation and differentiation dynamics at a key transition during erythropoiesis, identifying a role for RNA Pol II pausing in temporally coordinating the cell cycle and erythroid differentiation.

| S-EPMC10615711 | biostudies-literature

RNA Polymerase II pausing temporally coordinates cell cycle progression and erythroid differentiation.

Project description:The controlled release of promoter-proximal paused RNA polymerase II (Pol II) into productive elongation is a major step in gene regulation. However, functional analysis of Pol II pausing is difficult because factors that regulate pause release are almost all essential. In this study, we identified heterozygous loss-of-function mutations in SUPT5H , which encodes SPT5, in individuals with β-thalassemia unlinked to HBB mutations. During erythropoiesis in healthy human cells, cell cycle genes were highly paused at the transition from progenitors to precursors. When the pathogenic mutations were recapitulated by SUPT5H editing, Pol II pause release was globally disrupted, and the transition from progenitors to precursors was delayed, marked by a transient lag in erythroid-specific gene expression and cell cycle kinetics. Despite this delay, cells terminally differentiate, and cell cycle phase distributions normalize. Therefore, hindering pause release perturbs proliferation and differentiation dynamics at a key transition during erythropoiesis, revealing a role for Pol II pausing in the temporal coordination between the cell cycle and differentiation.

| S-EPMC10029049 | biostudies-literature

net

Project description:Drosophila melanogaster net, net, is differentially expressed in 18 experiment(s);

| FBgn0002931 | ExpressionAtlas

net

Project description:Drosophila melanogaster net, net, is expressed in 3 baseline experiment(s);

| FBgn0002931 | ExpressionAtlas