Project description:This genome-scale metabolic model (GEM) of Corynebacterium tuberculostearicum strain DSM 44922 (Taxon ID 38304) was initially built with CarveMe version 1.5.1 based on the genome assembly with NCBI accession GCF_013408445.1 and then underwent a series of careful semi-automatic and manual curation. It is the first model curated using the Python tool MCC for mass and charge curation.
Project description:We sequenced and analyzed the genome of a highly inbred miniature Chinese pig strain, the Banna Minipig Inbred Line (BMI). we conducted whole genome screening using next generation sequencing (NGS) technology and performed SNP calling using Sus Scrofa genome assembly Sscrofa11.1.
Project description:The severe inflammatory response of pulmonary epithelial cells is the main pathological feature of pneumonia caused by methicillin-resistant Staphylococcus aureus (MRSA). Zhenqi granules (ZQ), a compound herbal formula composed of Astragalus membranaceus and Ligustrum lucidum, has potential therapeutic effects on this inflammatory response, but its mechanism of action is still unclear. The study aims to explore the effect and mechanism of ZQ on the inflammatory response induced by establishing an in vitro model of MRSA-infected human pulmonary bronchial epithelial cells (MRSA-BEAS-2B). ZQ (10 mg/mL) had no inhibitory effect on MRSA or its biofilm, but it reduced the levels of LDH and c-di-AMP in MRSA-BEAS-2B cells. The mechanism is attributed to the downregulation of endoplasmic reticulum-related protein pathways, specifically involving a decrease in endoplasmic reticulum stress-related proteins such as STING and ERAdP; a decrease in inflammation pathway-related factors such as NF-ĸB, IFR3, NLRP3, and TLR4; and an increase in the STING antagonist RECON. These effects resulted in a reduction in the proinflammatory cytokines IFN-β, IL-1β, IL-6, and IL-18, thereby inhibiting pulmonary inflammation. ZQ attenuates the inflammatory response of pulmonary epithelial cells by inhibiting c-di-AMP-related endoplasmic reticulum stress during MRSA infection. This study provides a basis for the clinical application of ZQ and improves the development of treatment strategies for pulmonary MRSA infection.
Project description:Leishmania donovani WHO reference strain MHOM/IN/80/DD8 and Leptomonas seymouri isolates Ld 2001 and Ld39 were used for proteome analysis which were originally isolated from clinical cases of kala azar patients with different inherent antimonial sensitivities. Ld 2001 was Sb-S and Ld 39 was Sb-R. The genome sequencing of these isolates had confirmed co-infection with Leptomonas.
