Project description:The data set contains MS/MS data on teeth extracts for Ancient DNA teeth samples ran in both positive and Negative ionization modes
Project description:DNA methylation is a conserved epigenetic mark that is involved in many biological processes including the ripening process of various crop species bearing fleshy fruit, such as tomato, strawberry, and orange. The dynamics and importance of DNA methylation during grapefruit ripening, has however not yet been characterized. In this study, we observed an increase in DNA methylation level during grape berry ripening. The application of a DNA methylation inhibitor delayed ripening, consistent with an important role for DNA methylation in grape berries. In addition, we characterized the methylomes of the flesh and the skin the two main tissues of grape berry pericarp, as they present very distinct metabolic features. Results indicate that grape berry skin has a lower DNA methylation level than the flesh. Whereas in both tissues ripening is associated with an increase in DNA methylation, methylation patterns are clearly distinct between these two tissues as are the metabolic and transcriptomic profiles. Results indicated that the DNA methylation changes are associated with tissue specific metabolic modifications that occur during ripening, and involved in the synthesis of secondary metabolites.
Project description:DNA methylation is a conserved epigenetic mark that is involved in many biological processes including the ripening process of various crop species bearing fleshy fruit, such as tomato, strawberry, and orange. The dynamics and importance of DNA methylation during grapefruit ripening, has however not yet been characterized. In this study, we observed an increase in DNA methylation level during grape berry ripening. The application of a DNA methylation inhibitor delayed ripening, consistent with an important role for DNA methylation in grape berries. In addition, we characterized the methylomes of the flesh and the skin the two main tissues of grape berry pericarp, as they present very distinct metabolic features. Results indicate that grape berry skin has a lower DNA methylation level than the flesh. Whereas in both tissues ripening is associated with an increase in DNA methylation, methylation patterns are clearly distinct between these two tissues as are the metabolic and transcriptomic profiles. Results indicated that the DNA methylation changes are associated with tissue specific metabolic modifications that occur during ripening, and involved in the synthesis of secondary metabolites.