Project description:The goals of this study were to determine differential gene expression between Achromobacter xylosoxidans clinical isolate Ax 7 in a synthetic artificial sputum media compared to a rich media control (LB).
Project description:Exosomal miRNA expression profiling of annotated miRNAs was performed on mouse serum samples from four groups: ND+oil (normal), WD+oil (NASH), ND+CCl₄ (cirrhosis), and WD+CCl₄ (HCC).
Project description:we investigated how cyclic compressive loading (CCL) could impact MSCs using three-dimensional cultures in atelocollagen-based meniscal substitute (ACMS). We extracted MSCs from the meniscus, synovium, and articular cartilage, cultured them in three-dimensional cultures, and submitted to CCL for seven days. We then compared the transcriptomes of MSCs treated with and without CCL. Our RNA-seq analysis revealed that CCL induced significant transcriptome changes, significantly affecting chondrocyte-related genes, including SOX9, TGFB1, and PRG4 upregulation. CCL-induced transcriptional differentiation from meniscus progenitors toward mature meniscal cells.
Project description:RNA sequencing was performed on mouse liver tissue samples from four groups: ND+oil (normal), WD+oil (NASH), ND+CCl₄ (cirrhosis), and WD+CCl₄ (HCC).
