Project description:Identifying the differentially expressed genes of Riemerella anatipestifer CH-1 and CH-1_zntR under normal condition through RNA sequencing.
Project description:Identifying the differentially expressed proteins of Riemerella anatipestifer CH-1 and CH-1_zntR under TSB condition through proteomic analysis
Project description:Riemerella anatipestifer can cause infectious serositis in ducklings with high mortality. As a bacterial adhesin receptor, membrane cofactor CD46 plays a role in the process of bacterial adhesion to the host. Outer membrane protein (OMP) is necessary for Gram-negative bacteria to adhere, colonize and cause diseases. Therefore, we used duck CD46 protein (dCD46) as bait protein, and screened out the OMPs of Riemerella anatipestifer which can interact with dCD46 by pull-down test, and searched for the adhesion protein of Riemerella anatipestifer, which laid a foundation for the prevention of Riemerella anatipestifer.
Project description:Infectious serositis of ducks caused by Riemerella anatipestifer infection has brought great losses to poultry industry all over the world. Adhesion and invasion were important pathway of the infection of Riemerella anatipestifer. Gram-negative pathogens’ adhesion and invasion of host cells by means of outer membrane proteins was a common phenomenon. Here, by using LC-MS/MS, we determined the adhesins belonging to the outer membrane protein of Riemerella anatipestifer RA-YM strain.
