Dataset Information

Adhesins, which could adhere to the duck embryo cell that belongs to the outer membrane proteins of Riemerella anatipestifer RA-YM strain sequencing by mass spectrometry (LC-MS/MS)

ABSTRACT: Infectious serositis of ducks caused by Riemerella anatipestifer infection has brought great losses to poultry industry all over the world. Adhesion and invasion were important pathway of the infection of Riemerella anatipestifer. Gram-negative pathogens’ adhesion and invasion of host cells by means of outer membrane proteins was a common phenomenon. Here, by using LC-MS/MS, we determined the adhesins belonging to the outer membrane protein of Riemerella anatipestifer RA-YM strain.

ORGANISM(S): Riemerella Anatipestifer Ra-ym

SUBMITTER: Zutao Zhou

PROVIDER: PXD057236 | iProX | Sun Oct 27 00:00:00 BST 2024

REPOSITORIES: iProX

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Publications

PorV factor of the type IX secretion system and PosF porin act as adhesins in Riemerella anatipestifer infection.

Li Sen S Wang Yanhua Y Ning Congran C Yang Rongkun R Wu Yaxin Y Cheng Xu X Ren Keke K Huang Minghua M Liu Xiong X Zhou Naiji N Zhang Wanpo W Hu Sishun S Xiao Yuncai Y Li Zili Z Zhou Hongbo H Liu Zhengfei Z Zhou Zutao Z

Veterinary research 20250608 1

Riemerella anatipestifer infection is a critical disease that is a major threat to the poultry industry worldwide. The adhesion and invasion of host cells are key steps in the primary stages of bacterial infection. However, the outer membrane proteins that mediate these events in R. anatipestifer are poorly characterized. In this study, the PorV and PosF proteins, as well as the previously described OMP71 protein, were identified as important mediators of the adhesion and invasion of duck embryo ...[more]

PMID: 40484959

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Project description:Background: Marek’s disease (MD) is a highly contagious, lymphomatous disease of chickens induced by a herpesvirus, Marek’s disease virus (MDV) that is the cause of major annual losses to the poultry industry. MD pathogenesis involves multiple stages including an early cytolytic phase and latency, and transitions between these stages are governed by several host and environmental factors. The success of vaccination strategies has led to the increased virulence of MDV and selective breeding of naturally resistant chickens is seen as a viable alternative. While multiple gene expression studies have been performed in resistant and susceptible populations, little is known about the epigenetic effects of infection. Results: In this study, we investigated temporal chromatin signatures induced by MDV by analyzing early cytolytic and latent phases of infection in the bursa of Fabricius of MD-resistant and –susceptible birds. Major global variations in chromatin marks were observed at different stages of MD in the two lines. Differential H3K27me3 marks were associated with immune-related pathways, such as MAP kinase signaling, focal adhesion and neuroactive ligand receptor interaction, and suggested varying degrees of silencing in response to infection. Immune-related microRNAs, e.g. gga-miR-155 and gga-miR-10b, bore chromatin signatures, which suggested their contribution to MD-susceptibility. Finally, several members of the focal adhesion pathway, e.g. THBS4 and ITGA1, showed marked concordance between gene expression and chromatin marks indicating putative epigenetic regulation in response to MDV infection. Conclusions: Our comprehensive analysis of chromatin signatures, therefore, revealed further clues about the epigenetic effects of MDV infection although further studies are necessary to elucidate the functional implications of the observed variations in histone modifications. Total of 32 samples analyzed; 2 histone modifications - H3K4me3 and H3K27me3 x 2 chicken lines with varying resistance to MD - L63 and L72 x 2 time-points of disease progression - 5 and 10 days post infection x 2 conditions - infected and control x 2 biological replicates

Dataset Information

Adhesins, which could adhere to the duck embryo cell that belongs to the outer membrane proteins of Riemerella anatipestifer RA-YM strain sequencing by mass spectrometry (LC-MS/MS)

Publications

PorV factor of the type IX secretion system and PosF porin act as adhesins in Riemerella anatipestifer infection.

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