Project description:Genomic DNA from five strains, Aspergillus fumigatus Af71, Aspergillus fumigatus Af294, Aspergillus clavatus, Neosartorya fenneliae, and Neosartorya fischeri, were co-hybridized with that of Aspergillus fumigatus Af293 and compared.
Project description:Experimantal validation for the 3' end of poly(A)-RNA in Aspergillus fumigatus is currently lacking. Therefore we performed 3'RNA-End sequencing from 24-h mycelium grown in AMM at 37°C and 42°C with a library kit purchased from Eclipse Bioinnovations.
Project description:Experimantal validation for the 5' end of poly(A)-RNA in Aspergillus fumigatus is currently lacking. Therefore we performed 5'RNA-End sequencing from 24-h mycelium grown in AMM at 37°C and 42°C with a library prepration kit purchased from Eclipse Bioinnovations.
Project description:Aspergillus fumigatus is an important human pathogen and a leading fungal killer. This study aimed to determine the small RNA repertoire of A. fumigatus in conidia and mycelium grown for 24 or 48 hours in liquid culture.
Project description:Investigation of whole genome gene expression level changes in trichostatin A (TSA)-treated A. fumigatus Af293 compared to non-treated A. fumigatus Af293. Species: Aspergillus fumigatus; Strain: Af293; Type of array: Eukaryotic Expression (4plex, 2plex for a TSA-treated sample and 2plex for a non-treated sample); Technical replicates: Two per treatment.
Project description:Experimantal validation for the 5' and 3' ends of poly(A)-RNA in Aspergillus fumigatus is currently lacking. The dataset here was used a total RNA-Seq control for poly(A)-enriched RNAs of Aspergillus fumigatus in comparison with 5' and 3' End-Seq data.
Project description:Aspergillus fumigatus is an important human pathogen and a leading fungal killer. This study aimed to determine the tRNA fragment and tRNA half repertoire of A. fumigatus in wild-type conidia and mycelium grown for 24 or 48 hours in liquid culture.
Project description:Purpose: The goal of this study is to investigate the responses of HUVECs after the stimulation of conidia of A. fumigatus Methods: HUVECs were stimulated with conidia of Aspergillus fumigatus for 2 and 6 hours. Three biological repeats of stimulated cells or un-stimulated controls were send for RNA sequencing. Results: Using an optimized data analysis workflow, we mapped about 40 million sequence reads per sample to the human genome (build hg38) and identified round 80,000 transcripts in the HUVECs upon stimulation. Conclusions: Our resutls showed the detailed analysis of HUVECs transcriptomes upton conidia of Aspergillus fumigatus stimulation.
