Project description:The Malayan pangolin (Manis javanica), an unusual mammal that is a scale-covered, toothless specialist myrmecophage, is maintained primarily through captive breeding in China. Maintaining this species in captivity is a significant challenge partly because little is known about its behavior and reproduction. The molecular mechanisms of its digestive system play a key role in the feeding and dietary husbandry of pangolins in captivity. Here, we performed the first large-scale sequencing of M. javanica transcriptomes from three digestive organs—the salivary glands, liver, and small intestine—by using Illumina HiSeq technology- to provides useful genetic resources for future functional work that may be relevant for the maintenance of captive pangolins.
Project description:This study aimed to investigate the differences in the immunity of Manis javanica and Manis pentadactyla through proteomics and microbiotas conjoint analysis.The nasal mucus of 10 pangolins (weight 5.71 kg ± 0.96 kg) was collected from the Wildlife Rescue Center of Shenzhen (Shenzhen City) and Guangdong Province (Guangzhou City). The pangolins from two different Wildlife Rescue Center were all in a healthy condition, although their feeding diets were not completely consistent.
Project description:Background: Parasitic helminths influence the composition of the gut microbiome. However, the microbiomes of individuals living in helminth-endemic regions are understudied. The Orang Asli, an indigenous population in Malaysia with high burdens of the helminth Trichuris trichiura, display microbiotas enriched in Clostridiales, an order of spore-forming obligate anaerobes with immunogenic properties. We previously isolated novel Clostridiales that were enriched in these individuals and found that a subset promoted the Trichuris life cycle. In this study, we aimed to further characterize the functional properties of these bacteria.Results: Clostridiales isolates were profiled for their ability to perform 57 enzymatic reactions and produce short chain fatty acids (SCFAs) and hydrogen sulfide, revealing that these bacteria were capable of a range of activities associated with metabolism and host response. Consistent with this finding, monocolonization of mice with individual isolates identified bacteria that were potent inducers of regulatory T cell (Treg) differentiation in the colon. Comparisons between variables revealed by these studies identified enzymatic properties correlated with Treg induction and Trichuris egg hatching.
2024-05-17 | GSE262316 | GEO
Project description:Virus diversity, wildlife-domestic animal circulation and potential zoonotic viruses of small mammals, pangolins and zoo animals
Project description:Vibrio vulnificus is a marine zoonotic pathogen associated with fish farms that is considered a biomarker of climate change. Zoonotic strains trigger a rapid death of their susceptible hosts (fish or humans) by septicemia that has been linked to a cytokine storm in mice. A toxin called RtxA1 produced by the bacteria might play an important role in bacterial invasion and subsequent death by septic shock since animals infected with a mutant deficient in rtxA1 suffer from septicemia but do not die. The aim of this study was to globally analyze the early eel immune response in blood against V. vulnificus, as well as the role of the RtxA1 toxin on this interaction.
