Expression data from Minoxdil, Keratin and microsphere keratin in vivo
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA901424 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Gene expression profiling reveals a potential effect of microsphere keratin in stimulating hair growth in vivo Mice back was depilated and then treated with 1% milliQ, 1% minoxidil, 1% keratin, 1% microsphere keratin for 20 days. Then the skin was collected and RNA was extracted from thetreatd skin as explained in the following section. The microarray was conducted for two biological replicates
2022-11-15 | GSE217935 | GEO
Project description:Differential expression of keratin and keratin associated proteins in spontaneously mutated mice
| PRJNA911295 | ENA
Project description:Expression of genes encoding multiple keratin- and keratin-associated proteins varies between C57BL/6 and MRL/MpJ (superhealer) mice following digit amputation. We characterized changes in gene expression by microarray analysis of regenerating digits in these mice at various timepoints following surgical amputation. These changes were validated by quantitative rtPCR analyses. In this dataset, we include the expression data obtained from regenerating mouse digits following surgical amputation as well unamputated digits (total 24 samples). These data are used to obtain genes that are differentially expressed between the two groups of digits.
2013-07-01 | E-GEOD-26227 | biostudies-arrayexpress
Project description:Atopic dermatitis (AD) is a pruritic and inflammatory disorder characterized by elevated levels of thymic stromal lymphopoietin (TSLP). Pruritus is prevalent in epidermolysis bullosa (EB). Currently, epidermal barrier disruption is known to trigger TSLP upregulation, however, mechanisms controlling TSLP expression remain incompletely understood. Tslp levels were highly upregulated in the epidermis and in the serum of keratin-deficient mice. Cultured keratinocytes either lacking keratins or expressing the dominant K14p.Arg131Pro show highly increased TSLP. Re-expression of wild-type K14 normalized TSLP levels. We demonstrate that keratins regulate Tslp expression in parts through MEK1/2 activation. The finding that 8 out of 17 EBS patients show elevated TSLP serum levels supports a major role of keratins in TSLP regulation. Our data identify a novel, keratin-dependent and cell-intrinsic regulation of Tslp. Elevated TSLP levels likely explain the high prevalence of pruritus in EBS and additional forms of EB, suggesting TSLP as novel biomarker for pruritus in EB. MEK1/2, in addition to calcineurin inhibitors, might be suitable drugs to treat itch in EB. Investigation was carried out using Keratin deficient keratinocytes isolated from typeI keratin mice.
2016-03-26 | E-GEOD-79596 | biostudies-arrayexpress
Project description:Expression of genes encoding multiple keratin- and keratin-associated proteins varies between C57BL/6 and MRL/MpJ (superhealer) mice following digit amputation. We characterized changes in gene expression by microarray analysis of regenerating digits in these mice at various timepoints following surgical amputation. These changes were validated by quantitative rtPCR analyses.
2013-07-01 | GSE26227 | GEO
Project description:We investigated epidermis derived fibroblasts in neonatal skin at single cell-transcriptomic levels. We found that keratin 5 lineage mesenchymal cells mainly showed the transcriptomic signatures as dermal papilla and dermal sheath cells. Our data suggested that hair follicle dermal cells were originally derived from epidermis via epithelial to mesenchymal transition.
2025-04-30 | GSE280868 | GEO
Project description:Identification of Keratin 5 expression lineage fibroblasts in hair follicles
| PRJNA1180947 | ENA
Project description:Firmicutes and dermatophytes degrade recalcitrant insoluble keratins resistant to proteolysis. Such highly ordered peptide fibrils can be digested through proteolysis as well as sulfitolysis. However, its biological mechanism relevant to keratin degradation remains unclear. Here we present genome-wide multi-omics analyses to describe how the extremophilic bacterium Fervidobacterium islandicum AW-1degrades keratin under stress conditions. Physiological and biochemical evidence integrated with multi-omics data reveals a nutrient deprivation-induced metabolic shift, thereby increasing chemotactic persister formation for cellular adhesion to keratins and activating the membrane-associated keratinolysis. Quantitative dynamic gene expression and metabolite profiling confirmed that stringent response-induced flagella synthesis is a prerequisite for biofilm formation and that keratin degradation is responsible for swarming dispersal. This study illustrates the molecular details of keratinolysis, providing insight into the survivability of primordial bacteria coping with scarce nutrition relevant to recurrent infections with superficial pathogens.
2026-03-16 | PXD027063 | Pride
Project description:Approximately 30% of women diagnosed with ERα breast cancer relapse with metastatic disease following adjuvant treatment with endocrine therapies. The connection between acquisition of drug resistance and invasive potential is poorly understood. In this study, we demonstrate that the type II keratin topological associating domain (TAD) undergoes epigenetic reprogramming in cells that develop resistance to aromatase inhibitors (AI), leading to keratin 80 (KRT80) upregulation. In agreement, an increased number of KRT80-positive cells are observed at relapse in vivo while KRT80 expression associates with poor outcome using several clinical endpoints. KRT80 expression is driven by de novo enhancer activation by sterol regulatory element-binding protein 1 (SREBP1). KRT80 upregulation directly promotes cytoskeletal rearrangements at the leading edge, increased focal adhesion maturation and cellular stiffening, which collectively promote cancer cell invasion. Shear-wave elasticity imaging of tumors from prospectively recruited patients shows that KRT80 levels correlate with stiffer tumors in vivo. Collectively, our data uncover an unpredicted and potentially targetable direct link between epigenetic and cytoskeletal reprogramming promoting cell invasion in response to chronic AI treatment.
2019-05-15 | GSE125128 | GEO
Project description:Atopic dermatitis (AD) is a pruritic and inflammatory disorder characterized by elevated levels of thymic stromal lymphopoietin (TSLP). Pruritus is prevalent in epidermolysis bullosa (EB). Currently, epidermal barrier disruption is known to trigger TSLP upregulation, however, mechanisms controlling TSLP expression remain incompletely understood. Tslp levels were highly upregulated in the epidermis and in the serum of keratin-deficient mice. Cultured keratinocytes either lacking keratins or expressing the dominant K14p.Arg131Pro show highly increased TSLP. Re-expression of wild-type K14 normalized TSLP levels. We demonstrate that keratins regulate Tslp expression in parts through MEK1/2 activation. The finding that 8 out of 17 EBS patients show elevated TSLP serum levels supports a major role of keratins in TSLP regulation. Our data identify a novel, keratin-dependent and cell-intrinsic regulation of Tslp. Elevated TSLP levels likely explain the high prevalence of pruritus in EBS and additional forms of EB, suggesting TSLP as novel biomarker for pruritus in EB. MEK1/2, in addition to calcineurin inhibitors, might be suitable drugs to treat itch in EB.
2016-03-26 | GSE79596 | GEO